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Liquid phase chromatography analysis method of biuret in compound mixed fertiliser

A liquid chromatography analysis and compound fertilizer technology, which is applied in the field of liquid chromatography analysis of biuret in compound fertilizers, can solve the problems of inability to meet the accuracy requirements, low recovery rate of biuret, cumbersome operation steps, etc. To achieve the effect of improving the first-pass rate, cost-effective testing, and accurate analysis

Inactive Publication Date: 2011-09-14
CHEM TECH ACAD OF SHANDONG PROVINCE
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Problems solved by technology

After this method is verified by experiments, it is found that the pretreatment steps of this method are cumbersome, the analysis time is long, and because the ion exchange resin has a certain adsorption on biuret, the recovery rate of biuret is low, which cannot meet the requirements of the analysis. Measurement accuracy requirements

Method used

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  • Liquid phase chromatography analysis method of biuret in compound mixed fertiliser
  • Liquid phase chromatography analysis method of biuret in compound mixed fertiliser
  • Liquid phase chromatography analysis method of biuret in compound mixed fertiliser

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Embodiment

[0034] ①Experimental equipment:

[0035] 1. Ultrasonic cleaner; 2. High performance liquid chromatography: with ultraviolet detector; 3. Micro syringe: 10 μL ~ 50 μL 4. Needle filter: prepare a 0.45 μm water phase microporous filter membrane; 5. 5 μL quantitative loop.

[0036] ②Experimental reagents:

[0037] 1. Methanol (chromatographically pure);

[0038] 2. Potassium dihydrogen phosphate;

[0039] 3. Biuret: Weigh about 15g of biuret (chemically pure) into a beaker, add it to 500mL of 95% ethanol, heat to dissolve, filter while hot, concentrate the filtrate to 250mL, cool to 5°C, precipitate crystals, filter, Dry at 105°C for later use. There should be no impurity peaks under the conditions specified in 3.2.6.

[0040] 4. Phosphoric acid solution: 30g / L;

[0041] 5. Potassium hydroxide solution: 40g / L;

[0042] 6. Potassium dihydrogen phosphate solution: 10g / L;

[0043] Weigh 10 g of potassium dihydrogen phosphate and dissolve it in 1 L of water, adjust the pH to 6....

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Abstract

The invention discloses a method used for liquid chromatographic analysis on biuret in compound fertilizer, which is characterized in that reversed-phase liquid chromatography is adopted; the flowing phase is phosphate buffer solution with the pH value of 6.0; the proportion of carbinol and buffer solution is 5:95; the flowing speed is 1.2ml / min; the detection wavelength is 200nm; a chromatographic column can resist pure water phase; C18 filler is provided and the granularity of the C18 filler is 5 mum; the column length is 250mm and the internal diameter of the column is 4.6mm; the content of biuret in the harmful compositions of the compound fertilizer is calculated by an external standard method; the liquid chromatography quantitative analysis is carried out on the biuret contained in the compound fertilizer by an ultraviolet detector; when the consistency of the biuret is 0.01mg / ml to 0.2mg / ml, the biuret has good linear relationship with the linear correlation coefficient of morethan 0.999; the method requires no predisposal on the analyzed sample, thus reducing a plurality of analysis steps and shortening the analysis time; the method is exact and reliable and has economical detection cost.

Description

technical field [0001] The invention relates to a liquid chromatography analysis method for biuret in compound fertilizers. Background technique [0002] Biuret is the condensation product of urea at high temperature. Excessive absorption of biuret by plants will cause chlorophyll loss and hypoplasia of the body, which can inhibit the growth of crops, especially harmful to the germination of seeds and the growth of young roots. my country is a big country in the production of compound (compound) fertilizers, and it is also a big country in the use and import of fertilizers. The quality level of compound (compound) fertilizers has a great impact on agricultural production. Due to the problem of production process control, especially after some new processes and processes are applied to the production of compound (compound) fertilizers, the content of biuret in fertilizers is relatively high, and the phenomenon of seedling burning occurs from time to time. However, since ther...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 邱娟崔爱红韩书霞
Owner CHEM TECH ACAD OF SHANDONG PROVINCE
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