Bacterium culture medium of northern cordyceps wine

Abstract

The invention relates to a culture medium for Cordyceps militaris strains, which is characterized by consisting of 100 grams of wheat grains, 200 grams of fresh silkworm chrysalis, 2 grams of peptone, 5 grams of glucose powder, 1 gram of potassium dihydrogen phosphate, 0.5 gram of magnesium sulphate, 100 milligrams of VB1 and 150 milliliters of water. In a conical flask of 250 milliliters, the wheat grains and the fresh silkworm chrysalis are added into nutritive water consisting of the peptone, the glucose powder, the potassium dihydrogen phosphate, the magnesium sulphate, the VB1 and the water; the conical flask is sealed by a cotton plug; and the mixture is sterilized for 1 hour at a temperature of 120 DEG C, and then cooled to obatin the culture medium. The culture medium has the advantages of simple and easily obtained raw materials, and simple preparation and use. Through repeated tests for dozens of times, the strains do not degenerate after the Cordyceps militaris strains are cultured by the culture medium. By contrast, after degenerate strains are inoculated to the culture medium to generate strains, the strains are prepared into liquid strains to plant, so the culture medium can improve the yield effectively.

Description

technical field [0001] The invention relates to a culture medium of Cordyceps militaris. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris, has broad market prospects due to its comprehensive health care functions and unique pharmacological effects. The cultivation of Cordyceps militaris is generally to put the strains of Cordyceps militaris into liquid culture medium to make liquid strains, and then plant them. But when Cordyceps militaris is planted with the tissue separation method for 2-3 generations, the bacterial strain can degenerate, seriously affecting the yield, and even not growing fruiting bodies. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a culture medium for the strain of Cordyceps militaris, so that the degenerated strain of Cordyceps militaris can grow fruiting bodies through cultivation, so as to increase the planting yield. [0004] The culture medium of...

AI Technical Summary

Problems solved by technology

But when Cordyceps militaris is planted with tissue separation method for 2-3 generations, the strain will degenerate, which will seriously affect the yield, and even no fruiting bodies will grow.