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A group of Eriocheir sinensis allergen proteins and preparation thereof

A technology of Chinese mitten crab and allergen, applied in the field of natural bioactive substances and their preparation

Inactive Publication Date: 2009-07-15
JIANGNAN UNIV
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  • Claims
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Problems solved by technology

At present, the labeling and content limits of allergen ingredients in my country's food standards are still blank, and there are no detection reagents for food allergens in food safety monitoring, and the analysis and identification of allergens in Chinese mitten crab tissues And its separation and preparation have not been reported at home and abroad

Method used

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  • A group of Eriocheir sinensis allergen proteins and preparation thereof

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example 1

[0012] 1. Extraction of Chinese mitten crab tissue protein Remove the head shell from the fresh and live Chinese mitten crab, take the pure muscle tissue and homogenize it with a homogenizer, add acetone at a ratio of 1:15, put it in a refrigerator at 4°C to degrease, and use it every day Stir for 2 hours and change the acetone solution once. After 48 hours, filter out acetone, weigh after natural drying, add 15ml PBS (0.01mol / L, pH7.4) per gram of sample, extract at 4°C for 48 hours, stir for 2 hours every day during this period, and then at 4°C 5000rpm× Centrifuge for 15 minutes and collect the supernatant.

[0013] 2. Separation of protein components of Eriocheir sinensis Adjust the pH value of the crude protein solution to 7.4, and carry out segmental salting-out with 30%, 50%, 70%, and 90% ammonium sulfate. Slowly add ammonium sulfate under stirring to achieve the required saturation, then slowly stir for 1 hour, stand at 4°C for 4 hours, centrifuge at 12000rpm×15min at ...

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Abstract

The invention invents a group of mitten crab-derived proteins with allergen activity and a preparation process thereof, and belongs to the technical field of natural bioactive substances and preparation technologies thereof. The invention provides a group of proteins with the allergen activity in a tissue of a mitten crab, and the molecular weights of the proteins are respectively 76KDa, 66KDa, 60KDa, 53KDa, 48KDa, 41KDa, 35KDa, 34KDa and 29KDa. High-purity mitten crab allergen protein components with better immunobiologic activity can be obtained on the premise of operation according to the preparation process. Main mitten crab allergen protein components prepared according to the preparation process can be used for preparing diagnostic reagents and desensitizers of clinical crab allergy and preparing reagents for detecting crab food allergens.

Description

technical field [0001] The invention relates to a group of proteins with allergen activity derived from Chinese mitten crab and a preparation process thereof, belonging to the technical field of natural bioactive substances and their preparation. Background technique [0002] Chinese mitten crab (Eriocheir sinensis), commonly known as hairy crab, river crab, and hairy crab, used to be wild in rivers and lakes, but now it is mainly a species of artificial breeding. It is the most common and popular crustacean aquatic food in the aquatic food market in my country. , is also one of the common foods that cause food-borne allergic diseases. [0003] Allergic reactions caused by food are a large class of common food-borne diseases, and its clinical manifestations are various, ranging from skin allergies and urticaria in mild cases to diarrhea, vomiting, indigestion, fever, acute angioedema, etc. in severe cases In severe cases, shock or even death may occur. The World Food and Ag...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K1/36C07K1/18C07K1/16
Inventor 陈伟吴兴达李娟陈味味李雪頔汤鲁宏邓超刘平静黄海波
Owner JIANGNAN UNIV
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