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Core fragment of human ZHX2 transcription factor and application thereof

A technology of transcription factors and core fragments, applied in the field of genetic engineering, can solve the problem of less than 20% surgical resection rate

Inactive Publication Date: 2009-09-16
SHANDONG UNIV
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Problems solved by technology

However, limited by surgical indications, the surgical resection rate is less than 20%, and the postoperative recurrence rate is over 70%.
[0008] At present, using the eukaryotic expression vector of the ZHX2 gene as a drug for the treatment of HCC, through the expression of the core fragment of ZHX2 to regulate downstream genes such as AFP and GPC3, and inhibit the growth of liver cancer cells, has not been reported at home and abroad.

Method used

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  • Core fragment of human ZHX2 transcription factor and application thereof
  • Core fragment of human ZHX2 transcription factor and application thereof
  • Core fragment of human ZHX2 transcription factor and application thereof

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Embodiment

[0035] For the operation steps not specifically described in the examples, refer to the commonly used operation specifications in this field.

[0036] ●Construction of different truncated fragments of ZHX2

[0037] 1 Obtaining the target gene fragment

[0038] 1.1 Using the cDNA of pcDNA-ZHX2-HA as a template, perform PCR amplification according to the designed primers of different truncated fragments of ZHX2. Take a 0.2ml PCR reaction tube, use the cDNA of pcDNA-ZHX2-HA as a template, and use F1 / R1, F2 / R2 and F2 / R1 as primers respectively to amplify ZHX2(1-501AA)-HA, ZHX2(242-338AA) - HA, ZHX2(242-501AA) - HA gene. The sequence of F1 is shown in SEQ ID NO.3, the sequence of R1 is shown in SEQ ID NO.4, the sequence of F2 is shown in SEQ ID NO.5, and the sequence of R2 is shown in SEQ ID NO.3.

[0039] The reaction system was loaded as follows:

[0040] ZHX2-HA(1-501AA):

[0041] Reaction solution (2×Platinum Taq 12.5μl

[0042] PCR Master Mix)

[0043] Upstream...

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Abstract

The invention relates to a functional fragment of a human ZHX2 transcription factor and application thereof, which belong to the technical field of gene engineering. A core fragment of the human ZHX2 transcription factor has a nucleotide sequence shown in SEQ ID No.1; and protein encoded by the core fragment has an amino sequence shown in SEQ ID No.2. The invention also relates to an expression vector containing the core fragment and a construction method thereof, and application of products in inhibiting the growth of human liver cancer cells. The invention provides a way with attractive application prospect for treating hepatocellular carcinoma, and provides an evidence for the development of novel medicaments.

Description

(1) Technical field [0001] The invention relates to a core functional fragment of human ZHX2 transcription factor and application thereof, belonging to the technical field of genetic engineering. (2) Background technology [0002] Hepatocellular carcinoma (HCC) refers to tumors arising from liver cells or intrahepatic cholangiocytes. The incidence of HCC ranks fifth among all tumors in the world, and its case fatality rate ranks third. Hepatitis B virus (HBV) infection is an important risk factor for liver cancer. Clinical studies have found that 63.2% of patients with liver cancer are positive for HBsAg. my country is a high-incidence area of ​​HBV, about 170 million people are infected by HBV, so it is also a high-incidence area of ​​liver cancer. The mortality rate of liver cancer ranks first in the world, accounting for about 40% of the world's deaths. HCC has a short course of disease, rapid progression, and high mortality. Therefore, early detection and timely treatme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C07K14/435C12N15/79A61K48/00A61K38/17A61P35/00A61P1/16
Inventor 马春红史伟栾芳王金金申红玉
Owner SHANDONG UNIV
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