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Method for reducing aerial root of tissue culture seedling of hydrangea

A technique for hydrangea group and aerial rooting, which is applied in the field of plant tissue culture, can solve the problems of hydrangea aerial rooting and the like, and achieves the effects of low price, saving tissue culture costs, and simplifying transplanting procedures.

Inactive Publication Date: 2012-05-02
HUAZHONG AGRI UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for reducing the aerial roots of hydrangea tissue culture seedlings, to solve the difficult problem of producing aerial roots in the rooting process of hydrangea tissue culture seedlings, and to improve the transplanting survival rate of the regenerated plants of hydrangea
[0006] The present invention has done a comparative study on rooting and transplanting, a key step in the tissue culture of hydrangea, and uses perlite as a support instead of agar, combined with the design of rooting medium, to solve the problem of aerial rooting of hydrangea, thereby improving the stem base. Rooting rate and transplanting survival effect

Method used

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  • Method for reducing aerial root of tissue culture seedling of hydrangea
  • Method for reducing aerial root of tissue culture seedling of hydrangea
  • Method for reducing aerial root of tissue culture seedling of hydrangea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A: Add 50 mL of perlite to the culture bottle, then add 25 mL of liquid rooting medium (1 / 2 B5 basic medium + indole butyric acid (IBA) 0.2, 0.5, 1.0 mg / L + sucrose 10 g / L1, adjust pH to 5.8), sterilized by high pressure steam at 121°C for 20 minutes, and set aside;

[0051] B: Under sterile conditions, the terminal buds of hydrangea tissue culture seedlings (1-2cm in height, with more than 2 leaves) are inserted into the prepared rooting medium, and cultivated in the cultivation room for 30-50 days ( See figure 1 A, figure 2 ).

[0052] C: Take out the hydrangea tissue culture seedlings from the perlite, without cleaning and hardening, directly transplant the tissue culture seedlings into a substrate with peat soil: perlite volume ratio of 1:1;

[0053] The culture conditions of the above steps A and B are respectively: the light is 1500-2000lx, and the light time is 16h.d -1 , the culture temperature is 23-25°C;

[0054] The transplanting and cultivation conditi...

Embodiment 2

[0060] The cultivation program, cultivation conditions and cultivation management of the indoor potted field are the same as in Example 1.

[0061] The specific steps are:

[0062] A: Add 50mL of rooting medium to the culture bottle (1 / 2 B5 basic medium + IBA respectively set three ratios of 0.2, 0.5, 1.0mg / L + sucrose 10g / L + agar 7g / L, adjust the pH to 5.8) , sterilized by high-pressure steam at 121°C for 20 minutes;

[0063] B: Insert the terminal buds of hydrangea tissue cultured seedlings (1-2cm in height, with more than 2 leaves) into the rooting medium of step A under aseptic conditions in the ultra-clean workbench, and cultivate them in the culture room for 30- 50 days;

[0064] C: Take out the hydrangea tissue-cultured seedlings from the rooting medium added with agar in step B, wash the root culture medium, and directly transplant the hydrangea tissue-cultured seedlings into peat soil: perlite volume ratio of 1:1 In the substrate, the transplanting survival rate c...

Embodiment 3

[0070] The cultivation program, cultivation conditions and cultivation management in the cultivation room and the potted field are the same as in Example 1.

[0071] The specific steps are:

[0072] A: Add about 50 mL of perlite to the culture bottle, then add 0, 15 mL, 25 mL, and 35 mL of liquid rooting medium (1 / 2 B5 basic medium + IBA 0.5 mg / L + sucrose 10 g / L, adjust the pH to 5.8) , sterilized by high-pressure steam at 121°C for 20 minutes;

[0073] B: In the sterile environment of the ultra-clean workbench, put the terminal buds of hydrangea tissue cultured seedlings (1-2 cm in height, more than 2 leaves) into the rooting medium prepared in step A, and cultivate them in the culture room for 30 -50 days (see Figure 4 A, B, C).

[0074] C: Take out the hydrangea tissue culture seedlings from the perlite, without cleaning and hardening, directly transplant the hydrangea tissue culture seedlings into the substrate with a peat soil:perlite volume ratio of 1:1, and the tra...

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Abstract

The invention belongs to the technical field of plant tissue breeding, and in particular to a method for reducing the aerial root of hydrangea, aiming at solving the problem that the aerial root is generated in the tissue culture process of the hydrangea and improving the survival rate of tissue culture seedling of the hydrangea. The method is realized by the following steps: the tissue culture seedling of the hydrangea is taken as an experimental material and is inoculated in a liquid rooting medium (1 / 2B5+NAA or IBA 0.1-1.0mg / L+sugar 10g / L pH 4-7) of perlite (40-60ml)+25-35ml; after 30 days,no aerial root is generated in the tissue culture seedling, the rooting rate in a culture flask reaches 100%, and the transplanting survival rate reaches 100%. The method not only improves the transplant survival rate and simplifies the operating procedures of transplantation, but also saves the cost of tissue culture by using the perlite to replace agar and recycling the perlite.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for reducing aerial roots of hydrangea tissue culture seedlings, so as to solve the problem of aerial roots in the rooting process of hydrangea tissue culture seedlings and improve the transplanting survival rate of hydrangea tissue culture seedlings . Background technique [0002] Hydrangea macrophylla (Hydrangea macrophylla) Hydrangea family, Hydrangea genus, deciduous shrub, 1-2m high, colorful, large flowers, long flowering period, high ornamental value, is one of the important garden and indoor potted plants. In recent years, with the successful cultivation of miniature potted plants such as 'H.m.'SnowQueen' (H.m.'SnowQueen'), 'Red Bride' (H.m.'Blushing Bride') and other famous new varieties, miniature hydrangea has become more and more popular in domestic and foreign markets , its seedlings are in short supply. Tissue culture technology i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 王彩云黄丽莉刘峰李扬丽
Owner HUAZHONG AGRI UNIV
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