A kind of anticancer drug and its preparation method and application
An anticancer drug and a technology for preparation steps, which are applied in the field of anticancer drugs and their preparation, can solve the problems of low curative effect, unsatisfactory curative effect, and large toxic and side effects of cancer, and achieve the effect of improving clinical curative effect.
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Embodiment 1
[0014] Embodiment 1: anticancer drug and preparation method thereof
[0015] (1) Prescription: 1 part of Ganoderma lucidum, 1 part of Coix Seed, 2 parts of Tiger Eye Evergreen.
[0016] (2) Preparation method: Weigh 1 part of Ganoderma lucidum, 1 part of Coix Seed, 2 parts of Evergreen, dry and crush in the shade, soak and extract with 16 times the amount of 65% ethanol solution at 70°C for 4 times, 5 hours each time, recover the ethanol and concentrate into an extract, vacuum-dried to obtain an anticancer drug.
Embodiment 2
[0017] Embodiment 2: anticancer drug and preparation method thereof
[0018] (1) Prescription: 2 parts of Ganoderma lucidum, 2 parts of Coix Seed, 6 parts of Tiger Eye Evergreen.
[0019] (2) Preparation method: Weigh 2 parts of ganoderma lucidum, 2 parts of coix seed, 6 parts of evergreen, dry and crush in the shade, soak and extract with 18 times the amount of 70% ethanol solution at 75°C for 4 times, each time for 6 hours, and recover ethanol Concentrate into an extract and dry it in a vacuum to obtain an anticancer drug.
Embodiment 3
[0020] Example 3: Inhibitory Effect of Anticancer Drugs on Transplanted Lung Cancer
[0021] 1. Experimental animals and tumor strains
[0022] Male healthy C57BL / 6 mice, weighing 18-22 g, aged 6-8 weeks, were purchased from the Animal Experiment Center of our school; mouse Lewis lung cancer tumor strains were purchased from the Animal Experiment Center of Jiangsu Cancer Institute.
[0023] 2. Modeling and experimental methods
[0024] There were 60 C57BL / 6 mice, 10 were randomly reserved in proportion as the negative control group, and the remaining 50 were used for modeling. Prepare Lewis lung cancer tumor-bearing mice, peel off the tumor tissue in the vigorous growth period, and select a well-growing tumor of 1.5mm 3 Left and right, homogenate according to the ratio of tumor (g): normal saline (ml) 1:3, adjust the number of cells to 2×10 6 / ml. Select healthy mice, and inoculate 0.2 ml subcutaneously in the right axilla of each mouse under aseptic conditions. The entir...
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