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Method for evaluating Traditional Chinese medicine activity and mechanism based on holistic approach

A holistic view, the technology of traditional Chinese medicine, applied in the field of basic research on the effect of traditional Chinese medicine, to achieve the effect of broadening the research direction, simple operation and low cost

Active Publication Date: 2010-08-04
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this technology can only screen out isolated chemically active ingredients in traditional Chinese medicine, and cannot analyze the contribution of different ingredients in Chinese medicine to the overall activity, the interaction between ingredients, and the overall activity of all active ingredient groups.

Method used

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  • Method for evaluating Traditional Chinese medicine activity and mechanism based on holistic approach
  • Method for evaluating Traditional Chinese medicine activity and mechanism based on holistic approach
  • Method for evaluating Traditional Chinese medicine activity and mechanism based on holistic approach

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Detection and Evaluation of Active Components of Lycoris Alkaloids

[0036] Active ingredient peak knockout and capture:

[0037] The peaks that can interact with acetylcholinesterase (Acetylcholinesterase, AChE) in the Lycoris alkaloid extract were screened as the target peaks, that is, the active ingredient peaks, and were knocked out and captured.

[0038] Preparation of Lycoris alkaloids: Lycoris powder 5g, 10 times the volume of 70% methanol ultrasonic extraction twice, 30min each time, the combined extracts were concentrated to dryness, added 20mL 2% HCl to adjust the pH to 1, and filtered. The filtrate was adjusted to pH 10 with 1M NaOH. It was extracted three times with 2 times the volume of chloroform, and the chloroform layers were combined and concentrated to dryness. Dissolved in 10mL of methanol, centrifuged, injected, and analyzed by high performance liquid chromatography to obtain its fingerprint.

[0039] Preparation of AChE: Acetylcholinesterase freez...

Embodiment 2

[0056] Activity Detection and Evaluation of the Main Components of Huperzine Alkaloids

[0057] Principal component peak knockout and capture:

[0058] Determine the peak with a larger peak height in the Huperz alkaloid extract as the target peak, that is, the main component peak, and knock out and capture it.

[0059] Preparation of huperzine alkaloids: 5 g of huperzine powder, immersed in 10 times the volume of hydrochloric acid with a pH value of 3, ultrasonically extracted twice, 30 min each time. Combine the filtrates, adjust the pH value to 9 with ammonia water, and extract 3 times with 10 times the volume of chloroform. The chloroform layers were combined and concentrated to dryness. Dissolved in 10mL of methanol, centrifuged, injected, and analyzed by high performance liquid chromatography to obtain its fingerprint.

[0060] Determination of principal component peaks: sort the obtained huperzia fingerprint chromatographic peaks from large to small peak heights, sele...

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Abstract

The invention relates to the research field of traditional Chinese medicine effect material basis, particularly relating to a method for evaluating traditional Chinese medicine activity and mechanism based on holistic approach, which is characterized by comprising the following steps: passing traditional Chinese medicine extract through high efficient liquid to acquire a traditional Chinese medicine finger print; determining target peaks in the finger print; connecting the outlet pipeline of a high performance liquid chromatography detector with a six-way switch valve, and collecting single target peaks, target peak groups at random combination and residue component bundles after knockout of target peak groups by the switch valve; and performing pharmacodynamic evaluation on a collected sample to obtain the activity sequence of the whole activity of the target peak groups and single target peaks, contribution of each single target peak in the traditional Chinese medicine extract and mutual interaction relationship between components. The invention broadens the research direction of the traditional Chinese medicine effect material basis, and provides a research method of traditional Chinese medicine integral action mechanism with simple operation and low cost.

Description

technical field [0001] The present invention relates to the field of basic research on effect substances of traditional Chinese medicine, in particular to a method for evaluating the activity and mechanism of action of traditional Chinese medicine by knocking out and capturing the chemical components of traditional Chinese medicine based on the holistic view of traditional Chinese medicine. It is a method to study the influence of different components of traditional Chinese medicine on the overall activity and the interaction mechanism between the components. Background technique [0002] For a long time, the mainstream model of Chinese medicine research in China has mainly borrowed from the basic strategy of Western research on active ingredients of natural products: using pharmacological models (whole animals, isolated organs, cells and molecules, etc.) on the basis of chemical component extraction, separation and structure identification. Model) to test the biological act...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/86G01N30/06G01N30/02
Inventor 李萍周建良刘朋刘颖
Owner CHINA PHARM UNIV
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