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Method for fermenting and producing streptomycin from compound coat blood replenishing oral liquid dregs

A technology of compound red coat and oral liquid, which is applied in the field of microbiology, can solve the problems of low added value of feed protein, achieve the effects of improving the rate of bacterial cell fragmentation, high industrial added value, and saving raw material expenses

Inactive Publication Date: 2019-09-27
XIANGYU PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The applicant's previous patented technology "an environmentally friendly process for processing the drug residue of compound Hongyi Buxue oral liquid", through recycling the drug residue of compound Hongyi Buxue oral liquid, selects the combination of Enterococcus faecalis and Cellulomonas flavinogenes The method is to ferment the medicine dregs of Compound Hongyi Buxue Oral Liquid to degrade the medicine dregs to prepare bacterial protein. Among them, the protein content in the processed product is close to 30%, the cellulose degradation rate reaches about 40%, and the nutritional value is high, which is suitable for application in feed protein; however, the added value of feed protein is low, and the applicant continued to conduct further research on the processed product to enhance the application value

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The medicine dregs of Compound Hongyi Buxue Oral Liquid can be obtained with reference to "CN101411784A, a preparation process of Compound Hongyi Buxue Oral Liquid", which is as follows: take 20 g of fungus and add 16 times the weight of water, soak for 30 minutes, heat and slightly boil for 2 hours, Filter, add 10 times the weight of water to the dregs, heat and boil slightly for 1 hour, filter, collect the retentate 1, combine the filtrate, and concentrate to a density of 1.02; take 100g of peanut red coat, 100g of medlar, and 200g of jujube, Add water to decoct twice, add 10 times the weight of water for the first time, add 8 times the weight of water for the second time, heat and boil slightly for 1 hour each time, filter, collect the retentate 2, combine the decoction, and concentrate the filtrate to the density 1.07, add ethanol to make the weight ratio of ethanol in the concentrated solution reach 65%, refrigerate for 24 hours, filter, reclaim ethanol from the fil...

Embodiment 2

[0026] The Streptomyces culture medium prepared by using compound Hongyi Buxue Oral Liquid dregs is prepared according to the following process:

[0027] Prepare Enterococcus faecalis seed medium: yeast extract 5g, glucose 20g, ammonium sulfate 5g, potassium dihydrogen phosphate 0.5g, magnesium sulfate heptahydrate 0.01g, add water to 1000ml, adjust pH to 7.0-7.5 with ammonia water; 115- 118°C, steam sterilize for 20 minutes, then cool down to 33°C;

[0028] Insert Enterococcus faecalis into the Enterococcus faecalis seed culture medium, and cultivate with aeration, the ventilation volume is 0.2 VVM, the stirring speed is 100r / min, the cultivation temperature is 33°C, and the concentration of Enterococcus faecalis is 1×10 9 CFU / mL;

[0029] Configure Cellulomonas flavinum seed medium: glucose 30g, yeast powder 10g, dipotassium hydrogen phosphate 1g, magnesium sulfate heptahydrate 0.5g, sodium chloride 0.1g, ferrous sulfate heptahydrate 0.01g, stir well, add water to set Make...

Embodiment 3

[0037] The Streptomyces culture medium prepared by using compound Hongyi Buxue Oral Liquid dregs is prepared according to the following process:

[0038] Prepare Enterococcus faecalis seed medium: yeast extract 5g, glucose 20g, ammonium sulfate 5g, potassium dihydrogen phosphate 0.5g, magnesium sulfate heptahydrate 0.01g, add water to 1000ml, adjust pH to 7.0-7.5 with ammonia water; 115~ 118°C, steam sterilize for 20 minutes, then cool down to 33°C;

[0039] Insert Enterococcus faecalis into the Enterococcus faecalis seed culture medium, and cultivate with aeration, the ventilation volume is 0.2 VVM, the stirring speed is 100r / min, the cultivation temperature is 33°C, and the concentration of Enterococcus faecalis is 2×10 9 CFU / mL;

[0040] Configure Cellulomonas flavinum seed medium: glucose 30g, yeast powder 10g, dipotassium hydrogen phosphate 1g, magnesium sulfate heptahydrate 0.5g, sodium chloride 0.1g, ferrous sulfate heptahydrate 0.01g, stir well, add water to set Make...

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PUM

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Abstract

The invention belongs to the technical field of microorganisms and discloses a method for fermenting and producing streptomycin from compound coat blood replenishing oral liquid dregs. The method comprises the steps as follows: inoculating a streptomyces culture medium prepared from the compound coat blood replenishing oral liquid dregs with a streptomyces griseus seed culture solution in the inoculum size of 10%, and performing fermentation at 32-35 DEG C for 72 h or longer at 200-300 rpm and the ventilation amount of 0.3-0.4 vvm; during fermentation, supplementing glucose to control the concentration of glucose in a fermentation liquid not lower than 0.5%. The method uses the dregs and changes waste into wealth.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a method for producing streptomycin by fermenting medicinal dregs of compound Hongyi Buxue oral liquid. Background technique [0002] Streptomyces is the main strain producing streptomycin. Streptomycin is an antibiotic extracted from the culture solution of Streptomyces griseus, which belongs to the aminoglycoside basic compound and is the main drug for the treatment of Mycobacterium tuberculosis. Because the pain response of intramuscular injection of streptomycin is relatively small, it is suitable for clinical use. As long as the application target is selected properly and the dose is relatively appropriate, most patients can be injected for a long time. During the preparation of streptomycin by Streptomyces fermentation, culture medium is needed, and the culture medium generally uses components such as yeast extract and glucose, which are expensive; with the in...

Claims

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Application Information

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IPC IPC(8): C12P19/54C12R1/545
CPCC12P19/54
Inventor 林凡友吴士平郭增光刘倩
Owner XIANGYU PHARMA
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