Microbial formulation and method of using the same promote plant growth
A technology that promotes plant growth, microbial activator, used in fertilizers, applications, fertilizer mixtures, etc. made from biological waste
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Embodiment 1
[0054] Example 1: The effect of selected Trichoderma strains
[0055] In this example, a pot experiment was conducted to show that the selected Trichoderma microorganism can improve the efficiency of organic fertilizer. Sandy loam soil was used as a potting substrate, tomatoes (Lycopersicon esculentum) were used as test plants, and compost was used as organic fertilizer (0.5% w / w). The size of the flowerpot is 10 cm in diameter and height. Before transplanting tomato seedlings, four doses (ie, 10 1 , 10 4 , 10 6 And 10 7 CFU / g soil, expressed as doses 1, 2, 3 and 4) Trichoderma microorganisms were added to the potting mixture. The soil control sample was an uninoculated seedling, in which only organic fertilizer was used.
[0056] Measure plant biomass in the form of root dry weight and branch dry weight at harvest (38 days), such as Figure 1A with Figure 1B Shown. according to Figure 1A , The plant biomass of the roots of the inoculated seedlings (dose 2, 3 and 4) was signific...
Embodiment 2
[0058] Example 2: The effect of microbial blends
[0059] In this example, a pot experiment was performed to show that the selected microbial blend can improve the efficiency of the organic fertilizer. The microbial blend includes Bacillus polymyxa, Bacillus subtilis, Trichoderma harzianum, and Saccharomyces cerevisiae. Sandy loam soil was used as a potting substrate, tomatoes (Lycopersicon esculentum) were used as test plants, and compost was used as organic fertilizer (0.5% w / w). The size of the flowerpot is 10 cm in diameter and height. Three doses (ie, 10 2 , 10 3 And 10 4 CFU / g soil, expressed as dosages M1, M2, and M3) The microbial blend was added to the potting mixture. The control sample (Ctrl) was an uninoculated seedling, in which only organic fertilizer was used.
[0060] Measure the plant height, canopy, stem size, and chlorophyll index on the 41st day and record them in Tables 1 to 4, as Figure 2A to 2D Shown. according to Figure 2B , The canopy of inoculated see...
Embodiment 3
[0070] Example 3: Effect of microbial activator on Trichoderma strains
[0071] In this example, in a pot experiment, a Trichoderma microbe supplemented with different microbial activators was applied to tomato seedlings to show the effectiveness of the microbial activators. The test setup is similar to that described in Example 1. Table 5 summarizes the composition of the various activator formulations applied in this example, which include yeast autolysates, humus powder, and micronutrients with amino acids.
[0072] Table 5 Composition of activator preparation of Trichoderma
[0073]
[0074] Measure plant height, stem size, dry weight of branches and dry roots on day 35, such as Figure 3A to 3D Shown. As shown in the figure, compared with plants only treated with microorganisms (treatment number P0), microorganisms supplemented with yeast autolysates, humus powder and / or micronutrients (treatment numbers P0, P2-0.2, P3-8) , P4-0.05, P9-0.2) showed significantly higher values ...
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