Recombined Bt genes mvip3Aa11, mcry2Ab4, assortment of genes and application thereof

Abstract

The invention relates to recombined Bt genes mvip3Aa11, mcry2Ab4, an assortment of genes and application thereof, belonging to the technical field of biological prevention and control. The recombined genes mcry2Ab4 encode a protein having an amino acid sequence of SEQ NO 4 and has the nucleotide sequence of SEQ NO 3. The recombined genes mvip3Aa11 encode the protein with amino acid sequence of SEQ NO 2 and has the nucleotide sequence of SEQ NO 1. In the invention, the nucleotide compositions of the genes cry2Ab4 and vip3Aa11 are adjusted, so that the genes approach to the frequency of the codon of the plant gene and the encoded amino acid sequences are not changed; the assortments of recombined genes are expressed in the plant to obtain the resistance to the Lepidoptera. More importantly, the recombination of two proteins shows the remarkable cooperating effect and has very strong poisoning activity to resistant insects.

Description

technical field The invention belongs to the technical field of biological control, and in particular relates to gene combinations for expressing highly toxic proteins to lepidopteran pests, artificially synthesized sequences and applications thereof. Background technique Insect pests are one of the important factors causing agricultural production losses. According to statistics, the direct economic loss caused by insect pests to agricultural production is as high as 15% every year. Chemical pesticides have made important contributions to pest control and stable agricultural production. With people's awareness of the environmental hazards of chemical pesticides and the increasing awareness of environmental protection, in order to achieve sustainable agricultural development, the world is actively exploring new ways of pest control. Bacillus thuringiensis (Bt) is a Gram-positive bacterium widely distributed in soil, and the insecticidal crystal proteins (ICPs) produced by...

AI Technical Summary

Problems solved by technology

However, the insect resistance of these early Bt transgenic plants is very weak, it is difficult to detect mRNA transcription, and the protein expression level is very low

There are many reasons for the low expression of Bt genes in plants: for example, 1. The wild Bt gene is rich in AZ sequences, and the mRNA expressed in plants is unstable; 2. There may be eukaryotic gene inclusion in the wild Bt gene cleavage sites and transcription termination signal sequences, resulting in incomplete transcripts or abnormal processing of transcripts; 3. Microbes and plants have great differences in the frequency of codon usage in translation, which reduces translation efficiency; 4. True The 5'-UTR sequence of nuclear genes is very different from that of prokaryotic genes, and the 3' end of eukaryotic genes needs to be tailed to identify the signal sequence

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