Recombined Bt genes mvip3Aa11, mcry2Ab4, assortment of genes and application thereof

A gene and amino acid technology, applied in the field of biological control, can solve the problems of low expression, reduced translation efficiency, incomplete transcripts, etc.
CN101812467AActive Publication Date: 2010-08-25INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Publication Date
2010-08-25

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Abstract

The invention relates to recombined Bt genes mvip3Aa11, mcry2Ab4, an assortment of genes and application thereof, belonging to the technical field of biological prevention and control. The recombined genes mcry2Ab4 encode a protein having an amino acid sequence of SEQ NO 4 and has the nucleotide sequence of SEQ NO 3. The recombined genes mvip3Aa11 encode the protein with amino acid sequence of SEQ NO 2 and has the nucleotide sequence of SEQ NO 1. In the invention, the nucleotide compositions of the genes cry2Ab4 and vip3Aa11 are adjusted, so that the genes approach to the frequency of the codon of the plant gene and the encoded amino acid sequences are not changed; the assortments of recombined genes are expressed in the plant to obtain the resistance to the Lepidoptera. More importantly, the recombination of two proteins shows the remarkable cooperating effect and has very strong poisoning activity to resistant insects.
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Description

technical field The invention belongs to the technical field of biological control, and in particular relates to gene combinations for expressing highly toxic proteins to lepidopteran pests, artificially synthesized sequences and applications thereof. Background technique Insect pests are one of the important factors causing agricultural production losses. According to statistics, the direct economic loss caused by insect pests to agricultural production is as high as 15% every year. Chemical pesticides have made important contributions to pest control and stable agricultural production. With people's awareness of the environmental hazards of chemical pesticides and the increasing awareness of environmental protection, in order to achieve sustainable agricultural development, the world is actively exploring new ways of pest control. Bacillus thuringiensis (Bt) is a Gram-positive bacterium widely distributed in soil, and the insecticidal crystal proteins (ICPs) produced by...

Claims

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