44results about How to "Overcoming or delaying drug resistance" patented technology

The invention discloses a bactericide pesticide composite containing oligochitosan, which comprises a component A and a component B, wherein the component A is oligochitosan or a derivative of the oligochitosan; and the component B is one or several of active compounds. The pesticide composition can produce a synergistic action, reduce the dosage of a chemically synthetic bactericide, prevent or delay the medicament resistance of the bactericide, improve crop stress resistance and yield, regulate metabolic balance in plant bodies and improve quality of agricultural products, improve the application safety of the bactericide and is more safe to ecological environmental and human bodies.

The invention relates to an insecticidal composition containing fluorin inspect bisamide and monosultap. The insecticidal composition has strong insecticidal activity, is applied to the field of chemical protection of agricultural production plants, is mainly used for preventing and curing the harm of lepidoptera pests to crops such as paddy rice, vegetables, fruit trees, cotton, seeding plants, flowers, and the like, has excellent insecticidal effect, and is different from other traditional insecticidal compositions. The insecticidal composition is prepared by combining effective ingredientsof the fluorin inspect bisamide and the monosultap. The insecticidal composition comprises the following preparation raw materials by weight percent: 0.1% to 50% of the fluorin inspect bisamide, 5% to 90% of the monosultap and 8% to 90% of additive. The composition preparation is aqueous emulsion, microemulsion, wettable powder, suspending agent, water dispersible granule or water soluble granule. The additive comprises solvent and one of or the mixture of latent solvent, emulsifying agent, dispersant, humectant, anti-freeze agent, thickening agent, defoaming agent, disintegrant, adhesive andauxiliary carrier.

The invention relates to recombined Bt genes mvip3Aa11, mcry2Ab4, an assortment of genes and application thereof, belonging to the technical field of biological prevention and control. The recombined genes mcry2Ab4 encode a protein having an amino acid sequence of SEQ NO 4 and has the nucleotide sequence of SEQ NO 3. The recombined genes mvip3Aa11 encode the protein with amino acid sequence of SEQ NO 2 and has the nucleotide sequence of SEQ NO 1. In the invention, the nucleotide compositions of the genes cry2Ab4 and vip3Aa11 are adjusted, so that the genes approach to the frequency of the codon of the plant gene and the encoded amino acid sequences are not changed; the assortments of recombined genes are expressed in the plant to obtain the resistance to the Lepidoptera. More importantly, the recombination of two proteins shows the remarkable cooperating effect and has very strong poisoning activity to resistant insects.

The invention relates to a bacillus thuringiensis cry1Ca gene, expressed protein and application of the bacillus thuringiensis cry1Ca gene and belongs to the technical field of biological prevention and control. Insecticidal protein has amino acid sequence shown as SEQ ID NO.2. In a gene for encoding the insecticidal protein, preferably, the nucleotide sequence of the gene is shown as SEQ ID NO.1. The gene has high toxicity on lepidoptera pests and is applied to transformation of microorganisms and plants, so that the microorganisms and the plants have toxicity on the related pests, and drug resistance of the pests on engineering bacteria and transgenic plants is overcome and delayed.

The invention relates to a bacillus thuringiensi strain, a combination protein and application of the protein, and belongs to the technical field of biological prevention control. The preservation number of the bacillus thuringiensi strain IPPBI0TSUC1O12 is CGMCC No. 10783, wherein proteins ST1 and ST2 extracted from the strain have high toxicity to rice planthopper injurious pests, can be applied to transformation of the microorganism and the plant to enable the microorganism and the plant to show the toxicity to relative injurious pests, and prevent and slow down the insecticide resistance generation of the injurious pests to engineering bacteria and transgenic plants.

The invention relates to a reconstructed mvip3Aa11 gene and application thereof, belonging to the technical field of biological prevention and control. The code of the reconstructed mvip3Aa11 gene has the protein of the amino acid sequence shown in SEQ NO2, and the nucleotide sequence is shown in SEQ NO1. In the invention, by adjusting the nucleotide composition of the vip2Aa11 gene, the vip2Aa11 gene is close to the codon frequency of plant gene without changing the coded amino acid sequence, and the reconstructed gene combination is expressed in plant so as to obtain resistance against lepidoptera pests.

The invention relates to bacillus thuringiensis LTS290 as well as an insecticidal gene cry57Ab, an expression protein and application of the bacillus thuringiensis LTS290, and belongs to the technical field of biological control. The preservation number of the bacillus thuringiensis LTS290 is CGMCC No.10232. The insecticidal protein separated from the bacterial strain LTS290 has the amino acid sequence as shown in SEQ ID NO.2 and a gene for encoding the insecticidal protein; the nucleotide sequence of the gene is preferred as SEQ ID NO.1. The gene has a certain killing force for lepidoptera pests, shows toxicity to related pests when being applied to the conversion of microorganisms and plants, and can be used for overcoming and delaying generation of drug resistance of pests to the engineering bacteria and transgenic plants.

The invention relates to a bacillus thuringiensis FH21, an insecticide gene, an expression protein and an application thereof, and belongs to the technical field of biological control. The collection number of the bacillus thuringiensis FH21strain is CGMCC No.10090, four insecticide proteins are separated out from the strain, the strain has the amino acid sequences represented by SEQ IN No 2, 4, 6, 8, and gene for encoding the insecticide proteins, preferably, the nucleotide sequences of the gene are represented by SEQ ID No 1, 3, 5, 7. The strain and the gene have high toxicity to lepidoptera pests, is applicable for converted microorganisms and plants to show the toxicity to related pests, and the resistance to drugs of the pests to engineering bacteria and transgenic plants are overcome and delayed.

The invention relates to a modified mcry2Ab4 gene and its application, and belongs to the technical field of biological control. The modified mcry2Ab4 gene encodes a protein with an amino acid sequence as shown in SEQ ID NO4. The nucleotide sequence is as shown in SEQ ID NO3. By regulating the nucleotide composition of cry2Ab4 gene, its codon frequency is similar to that of a plant gene without changing the encoding amino acid sequence, and the modified gene composition is expressed in plant so as to obtain the resistance to lepidoptera insects.

The invention discloses a fungicidal composition containing 2-methyl sulfone-5-(2,4-dichlorophenyl)-1,2,3-oxadiazole and fluopicolide and application of the fungicidal composition. The active ingredients comprise 2-methyl sulfone-5-(2,4-dichlorophenyl)-1,2,3-oxadiazole and fluopicolide, the compound weight ratio of 2-methyl sulfone-5-(2,4-dichlorophenyl)-1,2,3-oxadiazole to fluopicolide is 1:30-30:1, and preferred weight ratio is 1:20-10:1. The fungicidal composition can be prepared into emulsifiable concentrate, wettable powder, oil-dispersible suspending agent, suspending agent, water dispersible granule, aqueous emulsion, microemulsion and granules. The fungicidal composition is reasonable in composition and good in fungicidal effect, and can achieve an obvious synergistic effect within the proportion range. The fungicidal composition has remarkable control effects on potato late blight and other various crop diseases.

The invention discloses an efficient insectofungicide containing beauveria bassiana and pyraclostrobin. Active ingredients of the product are beauveria bassiana and pyraclostrobin. Mass content of beauveria bassiana is 10-30%, and mass content of pyraclostrobin is 10-25%. The dosage form of the insectofungicide is an oil-suspended injection, wettable powder or water dispersible granules. The insectofungicide can be used for controlling plant diseases and insect pests for crops, and has an effect of treating plant diseases and insect pests for various crops, including main vegetable pests whitefly and diamondback moth and important vegetable diseases powdery mildew, downy mildew, late blight, early blight, anthracnose, etc. What's more important is that pyraclostrobin can enhance virulence of beauveria bassiana to diamondback moth.

The invention discloses a sterilizing composition comprising prochloraz and biological antibacterial substance HSAF (heat stable antifungal factor) and application of the sterilizing composition. Theprochloraz and the HSAF are main effective components of the sterilizing composition. The mass ratio of the prochloraz to the HSAF ranges from 1:10 to 10:1. The sterilizing composition has synergisticinteraction effect within the matching range, overcomes and delays drug resistance of bacteria, and has high sterilization speed, good prevention and treatment effect, long lasting period and low application cost. The sterilizing composition can be used for preventing and controlling various crop fungus and oomycete diseases, especially fungus diseases such as black spots, anthracnose, scabs andrust diseases of pear trees and apple trees, and has obviously better prevention and control effect than a single agent.