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Bacillus thuringiensi strain, expression protein and application of protein

A technology of Bacillus thuringiensis and strains, applied in the field of biological control, can solve problems such as environmental pollution

Active Publication Date: 2015-08-19
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] For the control of rice planthoppers, traditional chemical control methods are mostly used, such as chlorpyrifos, imidacloprid, malathion, pymetrozine, etofenprox, etc., which are likely to cause environmental pollution and the emergence of drug resistance
For herbivorous piercing-sucking mouthparts pests such as planthoppers, there is currently no highly effective insecticidal protein for biological control and transgenic plant development

Method used

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  • Bacillus thuringiensi strain, expression protein and application of protein
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  • Bacillus thuringiensi strain, expression protein and application of protein

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Embodiment Construction

[0034] The present invention will be further described in detail below in conjunction with the examples.

[0035] 1. Establishment and verification of bioassay system

[0036] Biometric method

[0037] Refer to the bioassay method reported by Fu et al. and improve it.

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Abstract

The invention relates to a bacillus thuringiensi strain, an expression protein and application of the protein, and belongs to the technical field of biological prevention control. Insecticide protein has an amino acid sequence shown in SEQ ID NO: 1 and a gene for encoding the insecticide protein, preferably the nucleotide sequence of the gene is as shown in SEQ ID NO: 2. The gene has high toxicity to rice planthopper injurious pests, can be applied to transformation of the microorganism and the plant to enable the microorganism and the plant to show the toxicity to relative injurious pests, and prevents and slows down the insecticide resistance generation of the injurious pests to engineering bacteria and transgenic plants.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to a Bt bacterial strain with high toxicity to rice planthopper agricultural pests, an insecticidal gene and a protein encoded by the gene. Background technique [0002] Rice planthoppers belong to the family Planthoppers of the order Homoptera. There are mainly three types of brown planthopper, white-backed planthopper and brown planthopper that harm rice. The brown planthopper and the white-backed planthopper are the most harmful ones. The white-backed planthopper is the main species in the early rice stage, and the brown planthopper is the main species in the later stage; the brown planthopper is the main species in the middle and late rice plants. SBPH seldom directly cause disasters, but can spread viruses of rice, wheat, corn and other crops. [0003] The long-winged adults of rice planthoppers can migrate long distances. Both adults and nymphs cluster on the lowe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N47/44A01P7/04C07K14/325C12N15/32C12R1/07
CPCA01N47/44A01N63/00C07K14/325C12N1/205C12R2001/075
Inventor 张杰束长龙王杨林克剑刘永磊耿丽丽王桂荣宋福平彭琦
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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