Bacillus thuringiensis fh21, insecticidal gene, expressed protein and application thereof

A technology of Bacillus thuringiensis and Bacillus aureus, applied in application, pesticide, genetic engineering and other directions, can solve the problems of increasing pesticide resistance and singleness of pests, and achieve the goal of expanding insecticidal spectrum, wide application prospects and delaying drug resistance. Effect
CN104673706BActive Publication Date: 2018-03-06NORTHEAST AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NORTHEAST AGRICULTURAL UNIVERSITY
Publication Date
2018-03-06

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Abstract

The invention relates to "bacillus thuringiensis FH21, insecticidal gene, expressed protein and application thereof", belonging to the technical field of biological control. A strain of Bacillus thuringiensis FH21, the preservation number of which is CGMCC No.10090, four insecticidal proteins were isolated from the strain, having amino acid sequences as shown in SEQ ID NO 2, 4, 6, and 8, and encoding the insecticidal protein The gene of insect protein, preferably its nucleotide sequence is shown in SEQ ID NO1, 3, 5, 7. The bacterial strain and the above-mentioned genes have high toxicity to lepidopteran pests, and can be used to transform microorganisms and plants, so that they can show toxicity to related pests, and overcome and delay the development of pesticide resistance of pests to engineering bacteria and transgenic plants.
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Description

technical field

[0001] The invention relates to the technical field of biological control, in particular, the invention further relates to a Bt insecticidal gene with high toxicity to lepidopteran agricultural pests and a protein encoded by the gene. Background technique

[0002] Bacillus thuringiensis (Bt) is a widely distributed Gram-positive bacterium, an entomopathogenic microorganism with strong toxicity to pests and no toxicity to natural enemies, and no toxicity to higher animals and humans. It is currently the most in-depth study and the most widely used microbial insecticide, and it is active against more than 3000 kinds of pests in 16 orders. Bt can form insecticidal crystal proteins (Insecticidal Crystal Proteins, ICPs), also known as δ-endotoxin (delta-endotoxin) during the sporulation stage, its shape, structure and size are closely related to its virulence [Schnepf.E, Crickmore .N,Van Rie.J.,Lereclus.D,Baum.J,Feitelson.J,Zeigler.D.R.,Dean.D.H.Bacillus thuringi...

Claims

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