Bacillus thuringiensis strains, combined proteins and applications thereof

Abstract

The invention relates to a bacillus thuringiensi strain, a combination protein and application of the protein, and belongs to the technical field of biological prevention control. The preservation number of the bacillus thuringiensi strain IPPBI0TSUC1O12 is CGMCC No. 10783, wherein proteins ST1 and ST2 extracted from the strain have high toxicity to rice planthopper injurious pests, can be applied to transformation of the microorganism and the plant to enable the microorganism and the plant to show the toxicity to relative injurious pests, and prevent and slow down the insecticide resistance generation of the injurious pests to engineering bacteria and transgenic plants.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular, the invention further relates to a Bt bacterial strain with high toxicity to rice planthopper agricultural pests, a combined protein and an application thereof. Background technique [0002] Rice planthoppers belong to the family Planthoppers of the order Homoptera. There are mainly three types of brown planthopper, white-backed planthopper and brown planthopper that harm rice. The brown planthopper and the white-backed planthopper are the most harmful ones. The white-backed planthopper is the main species in the early rice stage, and the brown planthopper is the main species in the later stage; the brown planthopper is the main species in the middle and late rice plants. SBPH seldom directly cause disasters, but can spread viruses of rice, wheat, corn and other crops. [0003] The long-winged adults of rice planthoppers can migrate long distances. Both adults and nymp...

AI Technical Summary

Problems solved by technology

[0006] For the control of rice planthoppers, traditional chemical control methods are mostly used, such as chlorpyrifos, imidacloprid, malathion, pymetrozine, etofenprox, etc., which are likely to cause environmental pollution and the emergence of drug resistance

For herbivorous piercing-sucking mouthparts pests such as planthoppers, there is currently no highly effective insecticidal protein for biological control and transgenic plant development

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