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Rhodococcus erythropolis and microbial inoculum and application thereof

A technology of rhodococcus and bacteria agent, which is applied in the field of rhodococcus, can solve the problems of difficult wastewater discharge, human and environmental hazards, etc., and achieve the effect of protecting health, protecting the ecological environment and being convenient to use

Active Publication Date: 2010-10-13
RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existence of chromium (VI) will not only cause direct harm to people and the environment, but also inhibit the ability of environmental biology to remove organic pollutants. It is difficult for general biological treatment to make the effluent of wastewater containing the above components meet the discharge standards.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: Obtaining and identification of Rhodococcus BZ-9

[0028] 1. Obtaining Rhodococcus BZ-9

[0029] Get 2g of activated sludge obtained from Shandong Binzhou tannery wastewater in December 2009 and add it to the basal salt liquid medium containing pyridine and phenol to cultivate for 7 days. After smearing and culturing for another 7 days, a single colony was picked, streaked, purified and stored.

[0030] Basal salt liquid medium composition: NaCl 1.0g, NH 4 NO 3 1.0g, K 2 HPO 4 1.5g, KH 2 PO 4 0.5g and MgSO 4 ·7H 2 O 0.2g, dilute to 1L with water, pH is about 7.0.

[0031] 2. Identification of Rhodococcus BZ-9

[0032] (1) Morphological characteristics: the bacteria are short rod-shaped.

[0033] (2) Physiological and biochemical characteristics: Gram stain positive bacteria (Gram stain positive); urease, catalase positive, oxidase, VP reaction and methyl red reaction negative; after growing on LB solid medium for 3 days, the colonies The siz...

Embodiment 2

[0038] Embodiment 2: Rhodococcus BZ-9 is to the degradation of different compounds in the basal salt liquid medium containing chromium (VI)

[0039] Pick a single colony of Rhodococcus BZ-9 and put it in 3ml LB liquid medium, shake it at 30°C and 165 rpm for 24 hours to obtain fresh bacterial solution.

[0040] Into 100ml of solution A (or solution B or solution C), insert 2ml of fresh bacterial solution, culture on a shaker at 30°C (170 rpm), and take a sample after 3 days (72 hours) to determine the degradation rate of the compound.

[0041] The preparation method of solution A: add phenol into the basal salt liquid culture medium, the final concentration of phenol is 100mg / L, add chromium (VI), make the final concentration of chromium be 20mg / L.

[0042] The preparation method of solution B: add 3-hydroxypyridine to the basal salt liquid medium, the final concentration of 3-hydroxypyridine is 100mg / L, add chromium (VI), so that the final concentration of chromium is 20mg / L....

Embodiment 3

[0047] Example 3: Degradation of different compounds by Rhodococcus BZ-9 in tannery wastewater

[0048] Pick a single colony of Rhodococcus BZ-9 and put it in 3ml LB liquid medium, shake it at 30°C and 165 rpm for 24 hours to obtain fresh bacterial solution.

[0049] Measure 100ml of chromium (VI)-containing wastewater, wherein the concentration of chromium (VI) is 2mg / l, and detect the added amount of phenol concentration therein. The concentration of phenol is 100mg / L.

[0050] Inject 2ml of fresh bacterial solution, mix well, place in a shaker at 30°C and 165 rpm for culture, and take a sample after 4 days (96 hours) to determine the degradation rate of the compound. The results are shown in Table 2.

[0051] Table 2 Removal of different compounds by Rhodococcus BZ-9 in tannery wastewater

[0052] substrate

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PUM

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Abstract

The invention discloses a rhodococcus erythropolis (rhodococcus sp.) BZ-9 which is preserved in the China General Microbiological Culture Collection Center with the accession number of CGMCC No.3695. The invention also discloses a microbial inoculum, the active component of which is the rhodococcus erythropolis BZ-9. The rhodococcus erythropolis BZ-9 and the microbial inoculum thereof have high-efficient degradation performance on various toxic organic substances as well as high degradation ability even under the condition of chromium (VI), and are applicable to treatment on coking wastewater, tanning wastewater, printing and dying wastewater and other organic wastewater containing phenol, pyridine and other toxic organic substances produced in the process of the production of the petrochemical industry.

Description

technical field [0001] The invention relates to a rhodococcus, a microbial bacterial agent produced by the rhodococcus, and the application of the rhodococcus and the microbial agent in degrading toxic organic substances in industrial wastewater containing chromium (VI) and treating wastewater. Background technique [0002] Pyridine, phenol and their derivatives are important chemical raw materials, and also the main components of industrial wastewater such as coking wastewater, printing and dyeing wastewater, and tannery wastewater. Studies have found that these compounds are harmful to humans and environmental organisms. The existence of chromium (VI) will not only cause direct harm to people and the environment, but also inhibit the ability of environmental biology to remove organic pollutants. It is difficult for general biological treatment to make the effluent of wastewater containing the above components meet the discharge standards. Microorganisms are often used as a...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C02F3/34C12R1/01A62D101/28A62D101/26C02F101/22C02F101/36C02F101/38C02F103/24C02F103/30C02F103/34
Inventor 孙纪全刘伟强吴晓磊赵晶晶徐莲周湧汤岳琴陈福明
Owner RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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