Chinese cabbage EST-SSR labeled primers and application thereof to identification of varieties

A technology for labeling primers and Chinese cabbage, which is applied in the field of molecular biology and can solve problems such as unsatisfactory development
CN101914618AActive Publication Date: 2010-12-15VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
Publication Date
2010-12-15

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Abstract

The invention relates to Chinese cabbage EST-SSR labeled primers and an application thereof to identification of varieties, belonging to the technical field of molecular biology. The invention provides sequences of a Chinese cabbage EST-SSR labeled primer BRE28, a Chinese cabbage EST-SSR labeled primer BRE121 and a Chinese cabbage EST-SSR labeled primer BRE131, and an application of the labeled primers to identification of varieties of Chinese cabbages. The amplified products of the EST-SSR primers of the invention are in parental complementary banding patterns which have obvious size difference, thus hybrids and parental inbred lines can be well distinguished, and different varieties of the Chinese cabbages can be identified. The amplified products among individuals in the same variety have consistent banding patterns.
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Description

technical field

[0001] The invention relates to a Chinese cabbage EST-SSR marker primer and its application in variety identification, belonging to the technical field of molecular biology. Background technique

[0002] Chinese cabbage (Brassica rapa L.ssp.pekinensis) is a Brassica plant in the family Brassicaceae, and it is an important vegetable in my country and East Asian countries. Variety differences directly affect the production and eating quality of Chinese cabbage, so the identification of Chinese cabbage varieties and purity identification are very important. The most reliable traditional testing method for seed purity is the GOT (grow-out test) method, which uses field planting to identify hybrids based on morphological characteristics. The biggest disadvantage of the GOT method is that it is time-consuming and labor-intensive, and it is easily affected by environmental and subjective factors. In recent years, isoenzymes and protein electrophoresis can be used ...

Claims

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