Chinese cabbage EST-SSR labeled primers and application thereof to identification of varieties

A technology for labeling primers and Chinese cabbage, which is applied in the field of molecular biology and can solve problems such as unsatisfactory development

Active Publication Date: 2010-12-15
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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Problems solved by technology

However, the development of Chinese cabbage

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  • Chinese cabbage EST-SSR labeled primers and application thereof to identification of varieties
  • Chinese cabbage EST-SSR labeled primers and application thereof to identification of varieties
  • Chinese cabbage EST-SSR labeled primers and application thereof to identification of varieties

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Embodiment

[0037] 1. Development of Chinese cabbage EST-SSR marker primers

[0038] The Chinese cabbage EST sequence used to develop primers comes from GenBank (http: / / www.ncbi.nlm.nih.gov), and as of October 31, 2007, there is a total of Chinese cabbage (B.rapa ssp.pekinensis) EST sequence 147217 indivual. In this study, 147217 Chinese cabbage EST sequences were used to develop SSR primers.

[0039] The downloaded Chinese cabbage EST sequence was pre-processed with reference to the method in Ge Jia et al. (see "Journal of Agricultural Biotechnology", No. 04, 2005, "Analysis of Chinese Cabbage Expressed Sequence Tag SSR Marker"), including removing the EST sequence. Existing vector sequence 5' end polyT and 3' end polyA sequence and sequences containing ambiguous bases, remove the sequence less than 100bp. Contigs were then constructed on the processed EST sequences using the SeqMan program (Lasergene software package, DNAStar Inc.). Use MISA software (http: / / www.pgrc.ipk-gatersleben....

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Abstract

The invention relates to Chinese cabbage EST-SSR labeled primers and an application thereof to identification of varieties, belonging to the technical field of molecular biology. The invention provides sequences of a Chinese cabbage EST-SSR labeled primer BRE28, a Chinese cabbage EST-SSR labeled primer BRE121 and a Chinese cabbage EST-SSR labeled primer BRE131, and an application of the labeled primers to identification of varieties of Chinese cabbages. The amplified products of the EST-SSR primers of the invention are in parental complementary banding patterns which have obvious size difference, thus hybrids and parental inbred lines can be well distinguished, and different varieties of the Chinese cabbages can be identified. The amplified products among individuals in the same variety have consistent banding patterns.

Description

technical field [0001] The invention relates to a Chinese cabbage EST-SSR marker primer and its application in variety identification, belonging to the technical field of molecular biology. Background technique [0002] Chinese cabbage (Brassica rapa L.ssp.pekinensis) is a Brassica plant in the family Brassicaceae, and it is an important vegetable in my country and East Asian countries. Variety differences directly affect the production and eating quality of Chinese cabbage, so the identification of Chinese cabbage varieties and purity identification are very important. The most reliable traditional testing method for seed purity is the GOT (grow-out test) method, which uses field planting to identify hybrids based on morphological characteristics. The biggest disadvantage of the GOT method is that it is time-consuming and labor-intensive, and it is easily affected by environmental and subjective factors. In recent years, isoenzymes and protein electrophoresis can be used ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 高建伟李利斌周新成刘立锋李化银张庶
Owner VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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