Method for identifying forsythia suspensa derivatives

A technology of forsythia derivatives and forsythia lignans is applied in the field of identification of natural medicinal plants, and achieves the effect of good identification effect

Active Publication Date: 2014-05-21
王顺才
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, for forsythia derivatives and related products, there are no reports and disclosures of technical content using IGD carbon NMR coupled fingerprint technology for identification

Method used

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  • Method for identifying forsythia suspensa derivatives
  • Method for identifying forsythia suspensa derivatives
  • Method for identifying forsythia suspensa derivatives

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0218] Example 1: Self-made Luanchuan Qingqiao lignan and phenylethanol glycoside extract IGD carbon NMR coupled fingerprint

[0219] (1) Preparation of characteristic extracts

[0220] Self-made Luanchuan Qingqiao lignan and phenylethanol glycoside extracts were prepared by refining and purifying the macroporous adsorption resin after alcohol extraction: weigh 20g of Luanchuan Qingqiao, add 8 times the volume of the medicinal material (160mL) Reflux extraction with 20% ethanol at 85°C for 3 times, 1 hour each time, filter, combine the filtrates, concentrate under reduced pressure until there is no alcohol smell, filter, put the filtrate on a D101 resin column (its mass is 50g), wash with water, 40 % ethanol was washed into the column (the volumes of water and ethanol in the column were 25 and 40 times the volume of the concentrated filtrate, respectively), and the 40% ethanol eluate was collected and evaporated to dryness under reduced pressure to obtain the homemade Luanchuan ...

Embodiment 2

[0234] Example 2: Commercially available forsythia extract IGD carbon nuclear magnetic resonance coupled fingerprint

[0235] (1) Feature extraction selection

[0236] The commercially available forsythia extract (Xi'an Kangpeiji Biotechnology Co., Ltd.) was directly selected as the characteristic extract.

[0237] (2) IGD carbon NMR fingerprint detection of commercially available forsythia extract

[0238] Take commercially available forsythia extract 30mg, dissolve in 0.5mL DMSO-d 6 In the process, IGD carbon-1-1C NMR spectrum detection was carried out to obtain the IGD carbon-1-1313 nuclear magnetic resonance spectrum fingerprint of the commercially available forsythia extract.

[0239] (3) Commercially available forsythia extract IGD carbon NMR fingerprint

[0240] 1) IGD carbon NMR fingerprint identification

[0241] In the IGD carbon NMR fingerprint of the commercially available forsythia extract, the characteristic signal of forsythia lignans is clearly shown: δ C ...

Embodiment 3

[0250] Example 3: Lignan and phenylethanol glycoside extract A (precipitation method) IGD carbon NMR coupled fingerprint of Luanchuan forsythia leaves

[0251] (1) Preparation of characteristic extracts

[0252] Luanchuan forsythia leaf lignan and phenylethanol glycoside extract A, which is prepared by precipitation and purification method after boiling: weigh 20g of forsythia leaf of Luanchuan, add water equivalent to 8 times the volume of the medicinal material (160mL) Reflux extraction at 95°C for 2 times, 1 hour each time, filter, dissolve the precipitate with MeOH after drying, filter, and dry the filtrate to obtain 1.56g of self-made Luanchuan leaf lignan and phenylethanol glycoside extract, which is used as the characteristic Extract.

[0253] (2) Self-made forsythia leaf lignans and phenylethanol glycosides extracts IGD carbon NMR fingerprint detection

[0254] Take Luanchuan Forsythia leaf lignan and phenylethanol glycoside extract A 30mg prepared in the previous st...

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Abstract

The invention relates to a method for identifying forsythia suspensa derivatives. The method comprises the following steps: (1) directly obtaining the forsythia suspensa derivatives as a forsythia suspensa lignans and phenylethanoid glycoside characteristic extract containing an active component group; (2) carrying out IGD (Integrated Graphics Driver) nuclear magnetic resonance spectroscopy fingerprint spectrum detection on the forsythia suspensa lignans and phenylethanoid glycoside characteristic extract so as to obtain peak intensities of characteristic peaks of a plurality of active components in the characteristic extract according to a fingerprint spectrum; and detecting the peak intensity of the characteristic peak of a standard reference corresponding to each active component in the same manner; (3) obtaining the absolute content of the standard reference by a quantitative analysis means; (4) calculating the content of each active component and the content of the active component group in the forsythia suspensa derivatives in a ratio of the peak intensities of the characteristic peaks and the absolute content. The method provided by the invention can be used for reflecting the lignans and phenylethanoid glycoside compounds contained in the forsythia suspensa derivatives and a ratio of the lignans compounds to the phenylethanoid glycoside compounds, so that the purpose of identifying the quality of the forsythia suspensa derivatives can be realized.

Description

technical field [0001] The invention belongs to the field of identification of natural medicinal plants, and in particular relates to a method for identifying derivatives of forsythia. Background technique [0002] Forsythia is one of the traditional Chinese medicines commonly used in clinical practice in China. It is the dried fruit of Forsythia suspensa (Thunb.) Vahl, a plant of the genus Forsythia in the family Oleaceae (Oleaceae). Qingqiao, harvested when the fruit is ripe and yellow in color is called Laoqiao. Forsythia belongs to the Lung, Heart, and Small Intestine Meridians. It is slightly cold in nature and bitter in taste. It has the effects of clearing heat and detoxification, reducing swelling and dispelling stagnation, and evacuating wind-heat [National Pharmacopoeia Committee. Pharmacopoeia of the People's Republic of China 2010 Edition Part One. China Medical Science and Technology Press , 159.]. Its active ingredients are mainly lignans and phenylethanol gl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N24/08G01N30/06G01N30/88
Inventor 董建军张海艳赵天增郭唯李晓陈玲范毅常霞王建军魏悦李倩
Owner 王顺才
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