Amygdalus communis EST (expressed sequence tag) microsatellite marker screening method

A technology of microsatellite markers and screening methods, which is applied in the field of screening and application of DNA markers in molecular biology, and can solve the problems of time-consuming, labor-intensive, slow development process, and high cost

Inactive Publication Date: 2011-04-27
ZHEJIANG FORESTRY UNIVERSITY
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AI Technical Summary

Problems solved by technology

SSR markers can be divided into genomic SSR and EST-SSR. The development process of traditional genomic SSR markers is slow and requires the construction of genomic libraries, identification and screening of repetitive sequence clones, sequencing, primer design, etc., which is not only time-consuming, labor-intensive, and costly also very high

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  • Amygdalus communis EST (expressed sequence tag) microsatellite marker screening method
  • Amygdalus communis EST (expressed sequence tag) microsatellite marker screening method
  • Amygdalus communis EST (expressed sequence tag) microsatellite marker screening method

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Embodiment Construction

[0012] The present invention is described in detail below in conjunction with the specific practices of microsatellite site screening and polymorphic marker determination:

[0013] 1. Data sources of almond ESTs and screening of microsatellite sequences

[0014] Search and download the almond EST sequence (format FASTA) from the database of the National Center for Biotechnology Information (NCBI) with the scientific name of almond, use Repeat Masker (http: / / www.repeatmasker.org) to remove repetitive sequences, and use Phrap (http: / / www.phrap.org) performed nucleotide sequence contig analysis and clustering to remove redundant nucleotide sequences, and then used the online program Websat (http: / / wsmartins.net / websat / ) to search for SSRs. The search criteria are: the repetition times of dinucleotide, trinucleotide, tetranucleotide, pentanucleotide and hexanucleotide repeat sequences are greater than or equal to 9, 6, 5, 4 and 3, respectively.

[0015] 2. Microsatellite marker p...

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Abstract

The invention relates to an Amygdalus communis EST (expressed sequence tag) microsatellite marker screening method. For the sequence of ESTs of Amygdalus communis disclosed by GeneBank, microsatellite retrieval software WebSat is used to excavate an EST microsatellite molecular marker and screen out two pairs of high-polymorphism Amygdalus communis microsatellite primer sequences. The Amygdalus communis microsatellite DNA marker is used for researches on the resource evaluation, the identification and the genetic diversity analysis of the Amygdalus communis, the construction of a genetic map, the molecular auxiliary selective breeding and the like, thereby being a reliable and effective molecular marker.

Description

【Technical field】 [0001] The invention belongs to the field of screening and application of DNA markers in molecular biology, and in particular relates to a screening method for almond EST (Express Sequence Tag, expressed sequence tag) microsatellite markers. 【Background technique】 [0002] Almond (Amygdalus commumis) has another name called almond apricot, belongs to the plant of the subgenus Amygdalus subgenus Amygdalus subfamily Rosaceae, is a kind of oil tree species with high economic value, and its kernel oil content is as high as 55-61%. There are 40 species of almonds in the world, and 6 species in China. Among them, almond wild resources are only naturally distributed in Kazakhstan and Xinjiang, my country. It is a precious relict deciduous forest tree species of the Tertiary and Miocene centuries in the Paleo-Mediterranean, known as "living fossil of plants". In order to conduct genetic analysis and genetic map construction of almond cultivars and wild resources, ...

Claims

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Application Information

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IPC IPC(8): C12N15/10C12Q1/68
Inventor 张智俊李亚玲吕志江罗淑萍
Owner ZHEJIANG FORESTRY UNIVERSITY
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