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Production method of petroleum hydrocarbon pollutant-degrading bacterium

A technology for petroleum hydrocarbon degrading bacteria and bacterial species activation, which is used in microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc.

Inactive Publication Date: 2011-06-22
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the current problems in the remediation of oil-contaminated soils, the present invention provides a low-cost production method for microbial agents suitable for intensified remediation of oil-contaminated soils

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0020] Embodiment 1: the production of petroleum hydrocarbon degrading bacterial agent

[0021] 1. Medium preparation:

[0022] 1) Strain preservation medium (solid, 1L): 2g peptone, 1g yeast extract, 5g sodium chloride, 20g agar, add sweet potato starch wastewater to 1L, pH 7.2;

[0023] 2) Strain activation medium (liquid, 1L): peptone 2g, yeast extract 2g, sodium chloride 5g, add sweet potato starch wastewater to 1L, pH 7.2;

[0024] 3) Seed culture medium (liquid, 10L): 1kg of oil-contaminated soil (i.e. oil sludge, petroleum hydrocarbon content above 2%), add sweet potato starch wastewater to 10L, pH 7.2;

[0025] 4) Fermentation medium (liquid, 200L): 10kg of oil-contaminated soil (i.e. oil sludge, petroleum hydrocarbon content above 2%), add sweet potato starch wastewater to 200L, pH 7.2;

[0026] All the above media were sterilized at 115°C for 20 min.

[0027] 2. Strain activation: pick petroleum hydrocarbon degrading bacteria and inoculate them on the strain prese...

Embodiment 2

[0031] Embodiment 2: the remediation effect of petroleum hydrocarbon degrading bacteria agent to petroleum-contaminated soil

[0032] The prepared petroleum hydrocarbon degrading bacterial agent is added into the petroleum-contaminated soil in an application amount of 3% by weight, and mixed evenly. Turn over once every 7 days, and measure the content of pollutants in the soil after 1 month. The content of alkanes decreased from 3950mg / kg to 1270mg / kg, a decrease of 68%; the content of aromatics decreased from 786mg / g to 391mg / kg, a decrease of 50%.

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Abstract

The invention relates to a method for producing Bacillus megaterium efficient petroleum hydrocarbon-degrading bacterium by utilizing sweet potato starch wastewater. belonging to the technical field of environmental biology. The petroleum hydrocarbon degrading bacteria adopts Bacillus megaterium which can separate petroleum-contaminated soil. The production method of the bacterium comprises the following five steps: preparing culture medium, activating strains, preparing liquid seeds, performing liquid state fermentation and preparing the bacterium, wherein sweet potato starch wastewater is used to provide the main carbon source and water of fermentation medium; and corn stalks are used as the carrier of microorganisms for preparing the solid bacterium. The technical scheme of the invention provides a new way for the source utilization of high concentration sweet potato starch wastewater; and the produced petroleum hydrocarbon-degrading bacterium can be used to perform efficient in-situ remediation of petroleum-contaminated soil and protect the ecological environment.

Description

Technical field: [0001] The invention belongs to the technical field of environmental protection, and relates to a preparation method of a microbial agent suitable for remediation of petroleum-contaminated soil. Background technique: [0002] In the process of oil exploitation and oil well construction, it is easy to cause leakage of petroleum hydrocarbons and pollute the soil. Saline-alkali soil itself has poor soil physical and chemical properties, and oil pollution further deteriorates the physical and chemical properties of soil and interferes with soil material circulation. In addition, pollutants such as polycyclic aromatic hydrocarbons contained in petroleum have a "three-to-one" effect, have a high ecological risk, and pose a potential threat to human health. Therefore, the remediation of oil-contaminated soil is an urgent task. [0003] At present, the remediation methods of oil-contaminated soil mainly include: extraction method, leaching method, composting metho...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/11
Inventor 彭霞薇王新新盛萱宜赵志瑞宿燕明白志辉
Owner BEIJING FORESTRY UNIVERSITY
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