Application of brusatol as chemotherapeutic drug synergist
A technology of brucelipidol and chemotherapeutic drugs, applied in the field of brucelipidol as a synergist of chemotherapeutic drugs, to achieve the effect of increasing growth inhibition
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Embodiment 1
[0027] Example 1: Determination of Nrf2 protein expression in non-small cell lung cancer A549, cervical cancer Hela, endometrial cancer Ishikawa and Spec-2, and breast cancer MDA-MB-231 cell lines
[0028] Experimental materials: Non-small cell lung cancer A549, cervical cancer Hela, endometrial cancer Ishikawa and Spec-2, breast cancer MDA-MB-231 cell lines were purchased from ATCC in the United States; bruceicol was isolated from Brucea javanica, NMR and MS Determine the structure; Nrf2 and GAPDH antibodies were purchased from Santa Cruz; cisplatin, carboplatin, etoposide, 5-FU and paclitaxel were purchased from Sigma.
[0029] experimental method:
[0030] (1) Cell treatment: Cells were treated with 1×10 6 The density of cells per well was seeded in 6-well plates, and treated with cholepicrol DMSO solution for 4 hours.
[0031] (2) Cell lysis: After the cell treatment is completed, wash the cells twice with ice-cold PBS to remove residual drugs, add SDS cell lysate 100 μl...
Embodiment 2
[0034] Example 2: Brucelipidol increases the effect of cisplatin, carboplatin, 5-FU, etoposide and paclitaxel on A549 cells
[0035] Experimental materials: the sources of cell lines, drugs, and culture medium are the same as those in Example 1.
[0036] Instrument: Roche Xcelligence cell real-time detection system.
[0037] Experimental method: Cells were seeded in Xcelligence special-purpose 16-well plates at a density of 8,000 per well. After the cells had adhered to the wall for 16-24 hours, they were pretreated with cholepicrol for 4 hours, and then chemotherapeutic drugs were added for co-cultivation. The cell growth index was recorded for 30 minutes and recorded for 72 hours.
[0038] Experimental results: The application of brucein alone temporarily inhibited the cells. With the prolongation of the treatment time, the cells in the brucein treatment group gradually resumed growth, while the growth index of the cells in the combined drug group was significantly smaller ...
Embodiment 3
[0039] Example 3: Brucelipid can increase the effect of cisplatin and carboplatin on Hela cells
[0040] Experimental material and experimental method are the same as embodiment 2.
[0041] Experimental results: Brucelipidin showed a synergistic effect with chemotherapeutic drugs in Hela cells, see image 3 .
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