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Crocodile collagen, and preparation and use thereof

A technology of collagen and collagen powder, which is applied in the fields of peptide/protein components, animal/human protein, pharmaceutical formulations, etc., and can solve problems such as incomplete development

Active Publication Date: 2011-06-29
BEIJING HONGYUAN AODA BIOTECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Alligator skin, scales, bones, palms and tail contain a lot of collagen, but it is not yet fully developed

Method used

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  • Crocodile collagen, and preparation and use thereof
  • Crocodile collagen, and preparation and use thereof
  • Crocodile collagen, and preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] Embodiment 1: crocodile collagen of the present invention

[0093] Take fresh crocodile skin, scrape off fat, wash, cut into small pieces, take crocodile scales, wash and remove impurities, scrape off fat, cut into shreds, take crocodile bones to remove muscles, break them into pieces; immerse the above substances in 10% Na 2 CO 3 After soaking for 4 hours, wash with water until neutral; then immerse in 4% HCl, soak for 4 hours, wash with water until neutral; after the above acid-base degreasing treatment, add 4% acetic acid with a solid-to-liquid ratio of 1:3 -Acetic acid solution, airtight, in a constant temperature water bath at 50°C, after 30min, add pepsin with an enzyme-to-substrate ratio of 2%, stir well, in a water bath at 37°C, after enzymolysis for 6 hours, coarse filter, centrifuge at 4000r / min for 30min, take Supernatant, set aside; add 4% acetic acid-acetic acid solution with a solid-to-liquid ratio of 1:3 for precipitation, airtight, place in a constant t...

Embodiment 2

[0094] Embodiment 2: crocodile collagen of the present invention

[0095] Take fresh crocodile skin, scrape off fat, wash, cut into small pieces, take crocodile scales to clean and remove impurities, scrape off fat, cut into shreds, take crocodile bones to remove muscles, break them into pieces; immerse the above substances in 12% Na 2 CO 3 After soaking for 3 hours, wash with water until neutral; then immerse in 5% HCl, soak for 3 hours, wash with water until neutral; after the above acid-base degreasing treatment, add 3% acetic acid with a solid-liquid ratio of 1:4 -Acetic acid solution, airtight, in a constant temperature water bath at 55°C, after 25min, add pepsin with an enzyme-to-substrate ratio of 3%, stir well, in a water bath at 36°C, after enzymolysis for 7 hours, coarsely filter, centrifuge at 3200r / min for 35min, take Supernatant, set aside; add 5% acetic acid-acetic acid solution with a solid-to-liquid ratio of 1:2 for precipitation, airtightly place it in a cons...

Embodiment 3

[0096] Take fresh crocodile skin, scrape off fat, wash, cut into small pieces, take crocodile scales and clean to remove impurities, scrape off fat, cut into shreds, take crocodile bones to remove muscles, break them into pieces; immerse the above substances in 6% Na 2 CO 3 After soaking for 5 hours, wash with water until neutral; then immerse in 3% HCl, soak for 5 hours, wash with water until neutral; after the above acid-base degreasing treatment, add 5% acetic acid with a solid-to-liquid ratio of 1:2 -Acetic acid solution, airtight, in a constant temperature water bath at 45°C, after 35min, add pepsin with an enzyme-to-substrate ratio of 1%, stir well, in a water bath at 39°C, after enzymolysis for 5 hours, coarse filter, centrifuge at 4800r / min for 25min, take Supernatant, set aside; add 3% acetic acid-acetic acid solution with a solid-to-liquid ratio of 1:4 for precipitation, seal it, place it in a constant temperature water bath at 55°C, and after 25 minutes, add pepsin ...

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Abstract

The invention discloses a preparation method of crocodile collagen, which can improve the added value of the production and processing of crocodiles, reduce environmental pollution and create high economic and social benefit. Pharmacodynamic experiments show that the crocodile collagen has obvious effects in terms of aging resistance, tissue repair, wound healing promotion and immunity enhancement.

Description

field of invention [0001] The invention relates to a crocodile collagen and a preparation method and use thereof. Background technique [0002] Collagen is a white, opaque, unbranched, fibrous protein with a molecule containing sugar and a large amount of glycine, proline and hydroxyproline, mainly found in animal tendons, ligaments, cartilage, skin and other connective tissues. It accounts for 25-33% of the total protein content in the human body or other animals. It has the functions of supporting organs and protecting the human body. Different types of collagen in different tissues are extracted from animals, and their functions and effects are also different. With the development of biochemistry, people's understanding of collagen has gradually improved. Now it has been confirmed that collagen, like immunoglobulin, is rich in diversity and specificity in tissue distribution, and is a functional protein related to the functions of various tissues and organs. . Alligato...

Claims

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Application Information

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IPC IPC(8): C07K14/78C12P21/06A61K38/39A61P39/06A61P17/02A61P37/02
Inventor 张博
Owner BEIJING HONGYUAN AODA BIOTECH DEV
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