A kind of hybridization seed production method of dianthus

A technology of hybrid seed production and carnation, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as inability to meet market demand, difficulty in breeding seeds, susceptibility to viruses, etc., to achieve less virus infection and better seed quality , the effect of good plant growth

Inactive Publication Date: 2011-11-30
DALIAN HAODI AGRI
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  • Application Information

AI Technical Summary

Problems solved by technology

Due to the male infertility of the female parent itself, it is very difficult to reproduce the seeds in the existing method, and it is easy to be infected with viruses, which affects t

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0032] detailed description Embodiment 1, take Chinese Dianthus male parent and American Dianthus female parent hybridization as example:

[0033] 1. Seedling cultivation

[0034] (1) Female seedling cultivation: choose the stem tip of American Dianthus as the isolated maternal tissue, and conduct tissue culture after the stem tip is sterilized. The tissue culture medium includes differentiation medium, rooting medium and subculture medium. Three Different culture media are prepared separately and used together. The formula of differentiation medium: MS plus BA1.0mg / L, NAA0.2 mg / L, sucrose 4000 mg / L, agar powder 5500 mg / L; the formula of rooting medium: MS plus NAA 0.2 mg / L, sucrose 4000 mg / L, agar powder 550 mg / L; subculture medium formula: big seedling MS plus IBA0.4 mg / L, BA0.5 mg / L, sucrose 4000 mg / L, agar Agar powder 5500 mg / L, seedling MS plus BA0.5 mg / L, NAA0.2 mg / L, sucrose 4000 mg / L, agar powder 5500 mg / L and MS plus BA1.0 mg / L, NAA0.4 mg / L L, sucrose 4000 mg / ...

Embodiment 2

[0054] Embodiment 2, take American Dianthus male parent and Chinese Dianthus female parent hybridization as example:

[0055] 1. Seedling cultivation

[0056] (1) Female seedling cultivation: choose the stem tip of Dianthus chinensis as the isolated maternal tissue, and conduct tissue culture after the stem tip is sterilized. The tissue culture medium includes differentiation medium, rooting medium and subculture medium. Three Different culture media are prepared separately and used together. The formula of differentiation medium: MS plus BA1.0mg / L, NAA0.2 mg / L, sucrose 4000 mg / L, agar powder 5500 mg / L; the formula of rooting medium: MS plus NAA 0.2 mg / L, sucrose 4000 mg / L, agar powder 550 mg / L; subculture medium formula: big seedling MS plus IBA0.4 mg / L, BA0.5 mg / L, sucrose 4000 mg / L, agar Agar powder 5500 mg / L, seedling MS plus BA0.5 mg / L, NAA0.2 mg / L, sucrose 4000 mg / L, agar powder 5500 mg / L and MS plus BA1.0 mg / L, NAA0.4 mg / L L, sucrose 4000 mg / L, agar powder 5500 mg...

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Abstract

A hybrid seed production method of Dianthus chinensis, in which the female parent adopts a combination of tissue culture and cuttings, and the male parent seedlings are directly sown, and after steps such as planting, field management, pollination, etc., the seeds are finally harvested. The invention has the advantages of high seed production yield, good seed quality, less virus infection, high production efficiency and the like.

Description

technical field [0001] The invention relates to a method for cultivating flowers, in particular to a method for producing hybrid seeds of dianthus. Background technique [0002] Dianthus is a perennial herbaceous plant of the genus Dianthus in the family Caryophyllaceae. The origin of Dianthus is China, and it is now widely cultivated all over the world. It also belongs to Dianthus barbata, that is, American Dianthus. Although Dianthus has been introduced and cultivated as an ornamental plant in the world, a large number of cultivars have been cultivated, and the Dianthus used in large-scale production is basically propagated by seeding, but it can also be propagated by division and cutting. Due to the male infertility characteristic of the female parent itself, it is very difficult to reproduce the seeds in the existing method, and it is easy to be infected with viruses, which affects the flowering period. In addition, for some flower breeding enterprises, the existing see...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G1/00A01G31/00
Inventor 辛学锐
Owner DALIAN HAODI AGRI
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