A method for screening drugs for the treatment of neurodegenerative diseases

A neurodegenerative and pharmaceutical technology, which is applied in the direction of material analysis and electrochemical variables of materials by measuring secondary emissions, can solve problems such as polar opposites, and achieve the effects of small impact, simple sample preparation, and high resolution

Inactive Publication Date: 2011-12-14
CENT SOUTH UNIV
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Problems solved by technology

Empirical exploration without theoretical guidance makes research in this area in an embarrassing situation where half the effort is half the effort or even the opposite is the opposite.

Method used

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  • A method for screening drugs for the treatment of neurodegenerative diseases
  • A method for screening drugs for the treatment of neurodegenerative diseases
  • A method for screening drugs for the treatment of neurodegenerative diseases

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Experimental program
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Embodiment Construction

[0027] 1. Aβ 42 Prepare a solution with a concentration of 25uM in PBS and incubate in a constant temperature water bath at 37°C for 2 days. The sample is prepared for morphology characterization with an atomic force microscope, and many Aβ oligomers and fibers can be obtained as shown in Figure 10h.

[0028] 0.25 mgAβ 42 Dissolve in 220 μL of NaOH (5 mM) solution, sonicate for 1 min, place at room temperature for 10 min, transfer to a centrifuge tube, perform high-speed centrifugation at 13,000 rpm for 30 min at 0°C, and pipette the supernatant Put it into a centrifuge tube and store it in the refrigerator at minus 20°C for later use. The stock solution concentration is 250 μM. When using, add HCl with the same amount and concentration as NaOH to neutralize it to become a neutral solution, and then dilute it with 10 mM PBS (PH=7.4) to obtain an Aβ solution with a concentration of 25 μM. Place them in a constant temperature water bath for incubation for 2 days.

[0029]2....

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Abstract

The invention relates to a method for screening drugs for treating neurodegenerative diseases and provides novel ways for screening the drugs for treating the neurodegenerative diseases by using the combination of an atomic force microscope (AFM) and electrochemistry. Potential drugs for treating the neurodegenerative diseases are screened through the topographic image change and the electrochemical working curve signal change of the potential drugs by using the combination of an AFM method and an electrochemical method. The method provided by the invention has the advantages that the operation is simple and convenient, the amount of consumption of reagents and samples is little, the screening cycle is short, the operation in a nearly physiological environment can be simulated and the result which is mutually verified by the two methods is true and reliable. The method for screening the drugs for treating the neurodegenerative diseases is suitable for strong-bond and low-bond systems and can be used for drug screening of natural products (such as traditional Chinese drugs), synthetic drugs and biological engineered drugs.

Description

technical field [0001] The present invention relates to a method for screening drugs for the treatment of neurodegenerative diseases, specifically a combination of atomic force microscopy and electrochemical methods, which can be very accurate, fast, direct, and A method for efficiently screening and evaluating potential drugs. Background technique [0002] Neurodegenerative disease, also known as neurodegenerative disease, is a disease state in which cells and neurons of the brain and spinal cord are lost[1,2]. The brain and spinal cord are made up of neurons that have different functions, such as controlling movement, processing sensory information, and making decisions. Cells in the brain and spinal cord generally do not regenerate, so excessive damage can be devastating and irreversible. Neurodegenerative diseases are caused by the loss of neurons or their myelin sheath [1,2], which worsens over time to lead to dysfunction. There are many neurodegenerative diseases, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N23/22G01N27/26
Inventor 张翼张瑞洁刘佳佳彭勇苏芳芳孙志方戴旋
Owner CENT SOUTH UNIV
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