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Method for extracting phenylethanoid glycoside active components from semenplantaginis

A technology of psyllium extract and phenylethanol glycosides, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of difficult extraction and separation, complex components, and low yield

Inactive Publication Date: 2012-01-11
BEIJING COLLAB PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The components of psyllium are complex, and many compounds have similar structures and properties. Therefore, it is difficult to extract and separate the active ingredients of phenylethanol glycosides from psyllium, and the yield is very low.

Method used

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  • Method for extracting phenylethanoid glycoside active components from semenplantaginis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Extraction: Fructus Plantaginis pulverizes, crosses 20 mesh sieves, takes by weighing 1000g and adopts ultrasonic extraction, adds 70% ethanol with 10 times of liquid-solid ratio, ultrasonic (frequency 80KHz) extracts 30min, repeats extraction 3 times, suction filtration, The extracts were combined, the ethanol was recovered and concentrated to 1000ml, cooled to room temperature, allowed to stand for 24 hours, centrifuged and filtered to obtain a clear filtrate.

[0027] (2) Refining: the above-mentioned filtrate is added to the pretreated HP-20 type macroporous resin column with a flow rate of 0.5BV / h, washed with water, 5%, 30% and 75% concentration of ethanol in sequence, HPLC For detection, collect the eluate rich in phenylethanol glycosides (30% ethanol eluted part), concentrate to a thick paste, and vacuum-dry to obtain the total phenylethanol glycosides extract of Semen Plantaginis. As determined by ultraviolet (UV) method, the content of phenylethanol glycos...

Embodiment 2

[0029] (1) Extraction: Semen Plantaginis is pulverized, passed through a 20-mesh sieve, weighed 1000g and extracted with 70% ethanol under reflux, adding 70% ethanol at a liquid-solid ratio of 4 times each time, refluxing for 2 hours for the first time, and refluxing for the second time Reflux for the third time for 1h. Suction filtration, combined extracts, recovered ethanol and concentrated to 1000ml, cooled to room temperature, let stand for 24h, centrifuged and filtered to obtain clarified filtrate.

[0030] (2) Refining: the above filtrate is added to the pretreated AB-8 type macroporous resin column with a flow rate of 1BV / h, washed with water, 5%, 30% and 75% concentration of ethanol in successive gradients, and detected by HPLC , collecting the 30% ethanol part rich in phenylethanol glycosides, concentrating to an extract, and drying in vacuum to obtain the total phenylethanol glycosides extract of Semen Plantaginis. As determined by ultraviolet (UV) method, the conte...

Embodiment 3

[0032](1) Extraction: Fructus Plantaginis is softened with steam, dried, pulverized, sieved, and 1000 g of Semen Plantaginis powder after weighing is taken, decocted and extracted with water, and deionized water is added with a liquid-solid ratio of 40 times, every Extract for 2 hours, repeat the treatment 3 times, filter the extracts, combine them, recover ethanol and concentrate to 1000ml, cool to room temperature, let stand for 24 hours, centrifuge and filter to obtain a clarified filtrate.

[0033] (2) Refining: Add the above-mentioned filtrate to the pretreated polyamide column at a flow rate of 1BV / h, wash with water, 10%, 50% and 75% concentration of ethanol in successive gradients, detect by HPLC, collect rich The 50% ethanol eluted part of the phenylethanol glycosides was concentrated under reduced pressure and vacuum-dried to obtain the total phenylethanol glycosides extract of Plantaginaceae. As determined by ultraviolet (UV) method, the content of phenylethanol gly...

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Abstract

The invention discloses a method for extracting phenylethanoid glycoside active components from semenplantaginis. The method comprises extracting by solvents and refining by a chromatography to obtain extract. The obtained extract contains phenylethanoid glycoside active components with a total content above 50%. The method is simple, is suitable for industrialized production, and has a prospect of industrial application.

Description

technical field [0001] The invention belongs to the technical field of natural medicines, and in particular relates to a method for extracting phenylethanol glycoside active ingredients from plantago seeds. Background technique [0002] Semenplantaginis (Semenplantaginis) is one of the traditional medicinal materials in my country. The use was first recorded in "Shen Nong's Materia Medica", and was listed as the top grade of medicine. [0003] Semen Plantago is Plantago depressa Willd. (Plantago depressa Willd.), also known as Plantago asiatica Linn. (Plantago asiatica Linn.), also known as the dried seeds of Plantago, which is distributed all over the country and has extensive resources . It has the effects of diuresis and stranguria, dampness and diarrhea, liver clearing and eyesight clearing, heat clearing and phlegm resolving. Psyllium contains a lot of mucus (polysaccharides) (see Tomoda Masashi, Yokoi Maemi, Ishikawa Kazuyo.Plant Mucilages.XXIX.[J].asiatica.J.Chen Ph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/18C07H1/08
Inventor 王珂赵大龙陈明霞王振辉
Owner BEIJING COLLAB PHARMA
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