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Chromogenic medium of coliform group and quick detection card thereof

A technology of chromogenic culture medium and coliform bacteria, which is applied in the field of microbiological inspection, can solve problems such as large visual side effects, restrictions on the promotion and application of medium, and troublesome result observation, and achieve the advantages of simple operation and reduction of false positives and false negatives probability and the effect of shortening the detection time

Active Publication Date: 2013-04-17
GUANGDONG DAYUAN OASIS FOOD SAFETY TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This test method is simple to detect Escherichia coli and is easy to observe, but the number of coliforms must be observed under ultraviolet light to observe the number of colonies of fluorescent bacteria. The visual side effects are particularly large, which limits the promotion and application of this medium to a certain extent

Method used

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  • Chromogenic medium of coliform group and quick detection card thereof
  • Chromogenic medium of coliform group and quick detection card thereof
  • Chromogenic medium of coliform group and quick detection card thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A coliform chromogenic medium, each 1000mL medium contains peptone 5g, yeast extract 50g, lactose 12.5g, sodium chloride 2g, sodium pyruvate 5g, SDS 0.5g, sodium dihydrogen phosphate 4g, 5-bromo -4-Chloro-3-indole-β-D-galactoside 0.001g, IPTG 1g.

[0040] Dissolve the above ingredients in water to 1000mL, heat to boil, sterilize at 121°C for 15min, and then use.

Embodiment 2

[0042] A coliform chromogenic medium, each 1000mL medium contains 8.7g peptone, 37.5g yeast extract, 25g lactose, 15g sodium chloride, 0.2g sodium pyruvate, 0.3g SDS, 0.1g sodium dihydrogen phosphate, 5-bromo-4-chloro-3-indole-β-D-galactoside 2g, IPTG 0.001g.

[0043] Dissolve the above ingredients in water to 1000mL, heat to boil, sterilize at 121°C for 15min, and then use.

Embodiment 3

[0045] A coliform chromogenic medium, each 1000mL medium contains 25g of peptone, 5g of yeast extract, 6.5g of lactose, 6.5g of sodium chloride, 0.1g of sodium pyruvate, 0.2g of SDS, 0.2g of sodium dihydrogen phosphate, 5-bromo-4-chloro-3-indole-β-D-galactoside 1g, IPTG 0.05g.

[0046] Dissolve the above ingredients in water to 1000mL, heat to boil, sterilize at 121°C for 15min, and then use.

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Abstract

The invention discloses a chromogenic medium of a coliform group and a quick detection card thereof. The medium contains peptone, yeast extract, lactose, common salt, sodium pyruvate, SDS, sodium dihydrogen phosphate, a specific enzyme chromogenic substrate and IPTG. The quick detection card consists of, in a bottom-up order: a negative film, a water absorption and moisture retention layer, a medium layer and a covering membrane, with the above medium adsorbed on the medium layer. The chromogenic medium of a coliform group and the detection card that are provided in the invention can detect coliform groups in drinking water, surface water or foodstuff, with short detection time and high efficiency. The chromogenic medium and the detection card of the invention can conduct qualitative detection to coliform groups, and simultaneously can carry out quantitative analysis. And a positive result has obvious color development and is convenient for observation, odds for emergence of false positive and false negative can be reduced, and the detection result is accurate.

Description

technical field [0001] The invention belongs to the field of microbiology testing, relates to a microbial chromogenic culture medium, and also relates to a quick detection card made with the chromogenic medium. Background technique [0002] As one of the important indicators of food hygiene quality, coliform bacteria can reflect the presence or absence of fecal contamination of the detected object on the one hand, and judge the degree of contamination of the sample according to the quantity. , preservation and other processes of hygiene, so it has a wide range of microbial significance. [0003] At present, there are many methods for the detection of coliform bacteria in imported and exported food in China. There are two methods stipulated in the National Food Safety Standard of the People's Republic of China for Coliform Bacteria Counting 4789.3-2010: MPN counting method and plate counting method. The MPN counting method is basically equivalent to the two-step method of th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/34C12Q1/04C12R1/19
Inventor 陈娟丽卢新
Owner GUANGDONG DAYUAN OASIS FOOD SAFETY TECH CO LTD
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