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Culture medium and culture method for high-density culture of Staphylococcus xylosus A2

A technology for high-density culture of Staphylococcus xylosus, applied in the field of microbial culture, can solve the problems of low yield, high production cost, long processing cycle and the like

Inactive Publication Date: 2012-12-26
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, as people's demand for high-quality meat products has gradually increased, it is of great significance to solve the problems of long processing cycle, low yield and high production cost of traditional fermented meat products that restrict their large-scale production

Method used

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  • Culture medium and culture method for high-density culture of Staphylococcus xylosus A2
  • Culture medium and culture method for high-density culture of Staphylococcus xylosus A2
  • Culture medium and culture method for high-density culture of Staphylococcus xylosus A2

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A high-density culture method of Staphylococcus xylosus A2, comprising the following steps:

[0040] a. Strain activation: Inoculate the frozen Staphylococcus xylosus A2 on the slope of MSA medium, cultivate it at 32°C until colonies appear, and then pick the lawn from the slope and insert it into another After colonies appear on the inclined plane, repeat the above operation and transfer three times in a row, so that the number of viable bacteria reaches 10 7 -10 8 cfu / mL to obtain activated strains;

[0041] The Staphylococcus xylosus A2 (Staphylococcus xylosus A2) is preserved in the General Microorganism Center of China Committee for Culture Collection of Microorganisms, and its strain preservation number is: CGMCC No.5049;

[0042] b. Preparation of culture medium:

[0043] First, weigh the following raw materials respectively: 0.5g lactose, 2g peptone, 0.2g yeast extract, 5g mushroom juice, 7.5g NaCl;

[0044] Then, above-mentioned raw material is dissolved in 1...

Embodiment 2

[0052] A high-density culture method of staphylococcus xylosus A2, is characterized in that, comprises the following steps:

[0053] a. Strain activation: Inoculate the frozen Staphylococcus xylosus A2 on the slope of MSA medium, cultivate it at 32°C until colonies appear, and then pick the lawn from the slope and insert it into another After colonies appear on the inclined plane, repeat the above operation and transfer three times in a row, so that the number of viable bacteria reaches 10 7 -10 8 cfu / mL to obtain activated strains;

[0054] The Staphylococcus xylosus A2 (Staphylococcus xylosus A2) is preserved in the General Microorganism Center of China Committee for Culture Collection of Microorganisms, and its strain preservation number is: CGMCC No.5049;

[0055] b. Preparation of culture medium:

[0056] First, weigh the following raw materials: 0.5g lactose, 2g peptone, 0.2g yeast extract, 5g mushroom juice, 7.5g NaCl;

[0057] Then, the above-mentioned raw material...

experiment example 1

[0065] The condition optimization of the high-density culture method of Experimental Example 1 Staphylococcus xylosus A2

[0066] The invention relates to a high-density culture method of Staphylococcus xylosus A2.

[0067] The high-density culture method step of described Staphylococcus xylosus A2 is as follows:

[0068] 1 Choice of carbon source

[0069] The liquid medium made by adding nine different carbon sources was studied, the activated Staphylococcus xylosus A2 was inoculated respectively, and cultured at a constant temperature of 32°C for 17 hours, the number of viable bacteria in the bacterial suspension was detected, the pH value was measured, and the carbon source was observed. its bacteriostatic effect. According to the results in Table 1, Staphylococcus xylosus A2 can grow normally and metabolize to produce acid in the medium supplemented with 9 kinds of carbon sources. The enrichment effect of the medium added with starch was significantly higher than that o...

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Abstract

The invention discloses a high-density culture method for Staphylococcus xylosus A2 and a culture medium used in the culture process. The stain is extracted and separated from fermented air-dried sausage, is identified as Staphylococcus xylosus and is named as Staphylococcus xylosus A2. The Staphylococcus xylosus A2 can be used for fermenting meat products. The invention further provides a high-density culture method for the stain, which comprises the following steps of: proliferating the culture medium, optimally controlling technological fermentation conditions, and preparing the high-density concentrated bacterial mud of the strain. A high-density culture of Staphylococcus xylosus with more than 2.07*10<11> cfu / g viable counts can be obtained through the culture method provided by the invention, and a basis for further utilizing the stain to prepare a direct throwing type Staphylococcus xylosus fermentation agent is founded.

Description

technical field [0001] The invention relates to a method for cultivating microorganisms, in particular to a high-density culture method for Staphylococcus xylosus. The invention belongs to the technical field of microorganism cultivation. Background technique [0002] Staphylococcus xylosus (Staphylococcus xylosus) is a very important strain in fermented meat products. It was first described by Schleifer Kloos in 1976 as a coagulase-negative staphylococcus (CNS) (Advances in Microbial Physiology, 1976, 13 : 245-292.). This bacterium is widely present in a variety of traditional meat products (Journal of Clinical Examination. 2001, 19(4): 226.), most of which can produce protease (Applied microbiology, 2002, 92(1): 158-164.) and Lipase (Food Microbiology, 2002, 19: 441-449.), has mild protein and fat decomposition ability, and produces some short-chain fatty acids, short peptides and amino acids through metabolism (Meat Science, 2006, 74: 281-288.) , to give meat products ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L1/31C12R1/44A23L13/00
Inventor 孔保华马宏慧刁新平
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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