Pneumatic oscillatory type cell culture system

A cell culture, oscillating technology, applied in the field of experimental systems, can solve the problems of large oscillation, unable to meet the experimental requirements, weak oscillation, etc., and achieve the effect of simple and ingenious structure and good oscillation effect.

Inactive Publication Date: 2013-09-11
广州市尤德生物科技有限公司
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current cell culture system can only provide the required heat from an external incubator at most, but the oscillation of the culture medium can only rely on mechanical oscillation, that is, use external means to vibrate and shake the culture flask of the culture system, or Directly extend the stirring device into the culture bottle and insert it into the culture medium for mechanical stirring. In fact, these vibration modes are either too weak to meet the experimental requirements; or too large to cause damage to the cells and tissues in the culture medium

Method used

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  • Pneumatic oscillatory type cell culture system

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Embodiment Construction

[0013] refer to figure 1 , a pneumatic oscillating cell culture system, which includes a constant temperature water bath 3, the constant temperature water bath 3 is provided with a culture bottle 2, and the culture bottle 2 is provided with a pumping device. Wherein the constant temperature water bath box 3 is an existing more conventional biological experiment water bath device, has a water circulation system and a heating system, can carry out a circulating water bath outside the culture bottle 2, and keep the culture solution in the culture bottle 2 at the required experimental temperature . The pumping device, as the name suggests, has the function of pumping air, that is, to ventilate the liquid to form water bubbles. When the water bubbles rise, the liquid will naturally roll and oscillate. Therefore, when the pumping device is installed in the culture bottle 2, it is only necessary to transfer the sample containing the experimental cells. The culture solution is inject...

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Abstract

The invention discloses a pneumatic oscillatory type cell culture system which comprises a constant temperature water bath box, wherein a culture bottle is arranged in the constant temperature water bath box and the culture bottle is internally provided with an air pump device. According to the invention, since the air pump device is arranged in the culture bottle, after the culture bottle is full filled with culture liquid containing experiment cells, the constant temperature water bath box can provide constant temperature water bath, the air pump device can pump air in the culture liquid, the pumped air naturally drives the culture liquid to turn over and oscillate, and because the oscillation is not forced oscillation caused by a mechanical stirring device, the cells can not be damaged. The pneumatic oscillatory type cell culture system has the advantages of simple and ingenious structure and good oscillation effect, can be widely applied to experiments of cell culture, fermentation, hybridization, biochemistry, enzyme and cell tissue and the like.

Description

technical field [0001] The invention relates to an experimental system used in biological experiments, in particular to a cell culture system. Background technique [0002] When performing experiments on cell culture, fermentation, hybridization, biochemistry, enzymes and cell tissues, it is usually necessary to create a cell culture environment, and for some microbial cells or colonies with special requirements, they need to survive in the culture medium. According to the requirements of the experiment, the culture medium must be maintained at a certain temperature and must be oscillated to keep cells and other experimental objects in a state of motion in the culture medium. Therefore, a cell culture system with temperature control and shaking functions is essential for these experiments. However, the current cell culture system can only provide the required heat from an external incubator at most, but the oscillation of the culture medium can only rely on mechanical oscil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00
CPCC12M23/08C12M27/16C12M29/06C12M41/22C12M41/40
Inventor 吴晓春
Owner 广州市尤德生物科技有限公司
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