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Wheat bran medium for culturing fruit bat fly in laboratory

A medium and laboratory technology, applied in the field of wheat bran medium for cultivating fruit flies in the laboratory, can solve the problems of insufficient production performance and easy mold, and achieve the effects of good production effect, low cost and strong mildew resistance

Inactive Publication Date: 2012-02-08
许乐乐 +3
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0004] In order to overcome the disadvantages of insufficient production performance and easy mold of the existing corn culture medium, the present invention provides a new type of fruit fly culture medium, wheat bran culture medium

Method used

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  • Wheat bran medium for culturing fruit bat fly in laboratory
  • Wheat bran medium for culturing fruit bat fly in laboratory

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Experimental program
Comparison scheme
Effect test

Embodiment approach

[0014] The pre-preparation method is:

[0015] (1) The composition of the wheat bran medium is 10 grams of wheat bran, 10 grams of brown sugar, 1.2 grams of agar, 1.2 grams of yeast, and 0.15 grams of benzoic acid. (2) Weigh enough components and put all the components into a 1000ml beaker; (3) Add 100ml of tap water to the beaker and stir slightly; (4) Put the beaker into 50 L pressure steam to extinguish (5) Set the parameters of the pressure steam sterilizer at 121°C for 20 minutes; (6) Take out the culture medium after sterilization, and put it in the room for 5 minutes before packaging.

[0016] The post preparation method is:

[0017] (1) Take an appropriate amount of glass test tubes (25×200 mm) and medical absorbent cotton, and put them in a blast drying oven at 120°C for 2 hours for dry heat sterilization; (2) Pour the sterilized culture medium into each tube 20 ml glass test tube; (3) place the glass tube at an angle (at a 30-degree angle to the tabletop) to form a...

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Abstract

A wheat bran medium for culturing fruit bat fly in laboratory comprises 10g of wheat bran, 10 g of brown sugar, 1.2 g of agar, 1.2g of yeast and 0.15g of benzoic acid. A preparation method of the medium comprises steps of: dissolving the components in 100 ml of tap water and carrying out an autoclaving; taking glass test tube (25*200 mm) treated with dry heat sterilization (120 DEG C for 2h); subpackaging 20ml of medium into each test tube; cooling to a room temperature until the medium being solid gel; and putting fruit bat fly in the medium, wherein a test tube plug is absorbent cotton treated with dry heat sterilization. The easily prepared medium has low costs, good production effect and strong mildewing resistance, and is convenient for genetics and development biology researches on fruit bat fly.

Description

technical field [0001] The invention relates to a wheat bran medium for cultivating fruit flies in a laboratory, which is used as the food of the fruit flies to enable the fruit flies to survive for a long time, so as to facilitate the research on the aspects of genetics and developmental biology of the fruit flies. Background technique [0002] Drosophila feeds on yeast, and any fermentable substrate can be used to raise fruit flies. Drosophila is easy to raise, but in the process of laboratory cultivation, it is very important to choose a medium that has good growth effect, good anti-mold effect, and is suitable for experimental operation of Drosophila. In the previous genetic experiments, the fruit flies were fed with corn flour medium according to the formula in "Genetics Experiment" (Second Edition, 1987) edited by Liu Zudong and Jiang Shaohui. However, the preparation is complicated, and the cultivation effect is not good, and the fecundity and eclosion time of fruit ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A01K67/033A23K10/30A23K50/90
Inventor 许乐乐王璐王且鲁葛康李永芹彭金法刘敏孙峰
Owner 许乐乐
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