Microalgae culturing device and microalgae culturing method

A technology of microalgae cultivation and culture solution, applied in the direction of microorganism-based methods, enzymology/microbiology devices, biochemical cleaning devices, etc., can solve the problems of difficult engineering and short photosynthesis time, and achieve gas-liquid mass transfer The effect of improved driving force, energy saving, and easy amplification

Inactive Publication Date: 2012-03-21
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the light absorption and photosynthesis time of algal cell photosynthesis is very short, gen

Method used

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  • Microalgae culturing device and microalgae culturing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Two ellipse-shaped open track pools (6.44 square meters of culture area) with a length of 3.8 meters and a width of 1.9 meters were used to cultivate Nanochloropsis outdoors for comparison and illustration.

[0036] One of the runway pools has a liquid filling height of 0.05m (total volume 360 ​​liters), and one side of it is connected to a liquid circulation pump through a pipeline. , the bottom of the cylindrical storage tank with a liquid capacity of 3.6 cubic meters, and the bottom of the culture solution storage tank is fed with air / CO 2 Mixed gas (5% CO 2 , v / v), the ventilation volume is 0.3VVM, and the gauge pressure of the storage tank is maintained at 1kg / cm 2 . From the top of the culture liquid storage tank, the culture liquid flows into the other side of the open runway pool through the pipeline. Control the flow rate of the water pump to 10 cubic meters per hour to keep the average residence time of the culture solution in the raceway pool at 120 second...

Embodiment 2

[0040] Two elliptical open track pools with a length of 3.8 meters and a width of 1.9 meters are used for outdoor cultivation of Nanochloropsis. One of the open runway pools has a liquid filling height of 0.05m (total volume 360 ​​liters), and one side of it is connected with a liquid circulation pump through a pipeline. 2.5 meters, the bottom of the cylindrical storage tank with a liquid capacity of 1.1 cubic meters, and the bottom of the culture solution storage tank is fed with air / CO 2 Mixed gas (5% CO 2 , v / v), the ventilation volume is 0.3VVM, and the gauge pressure of the storage tank is maintained at 1kg / cm 2 . From the top of the culture liquid storage tank, the culture liquid flows into the other side of the open runway pool through the pipeline. Control the flow rate of the water pump to 20 cubic meters per hour to keep the average residence time of the culture solution in the raceway pool at 60 seconds. Stop the circulation at night, and pump all the culture fl...

Embodiment 3

[0044] Two elliptical open track pools with a length of 3.8 meters and a width of 1.9 meters are used for outdoor cultivation of Nanochloropsis. One of the open runway pools has a liquid filling height of 0.05m (total volume 360 ​​liters), and one side of it is connected with a liquid circulation pump through a pipeline. 2.5 meters, the bottom of the cylindrical storage tank with a liquid capacity of 1.1 cubic meters, and the bottom of the culture solution storage tank is fed with air / CO 2 Mixed gas (5% CO 2 , v / v), the ventilation volume is 0.3VVM, and the gauge pressure of the storage tank is maintained at 1.6kg / cm 2 . From the top of the culture liquid storage tank, the culture liquid flows into the other side of the open runway pool through the pipeline. Control the flow rate of the water pump to 20 cubic meters per hour to keep the average residence time of the culture solution in the raceway pool at 60 seconds. Stop the circulation at night, and pump all the culture ...

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Abstract

The invention discloses a microalgae culturing device, which comprises an open runway pool, wherein the open runway pool is provided with a liquid outlet; the liquid outlet is connected with a liquid circulating pump, and is connected with a liquid inlet of a culturing liquid storage tank through the liquid circulating pump; the top of the culturing liquid storage tank is provided with a liquid outlet pipeline, so that a culture fluid can flow back into the open runway pool through the pipeline; and the bottom of the culturing liquid storage tank is provided with a gas distributor for introducing various CO2-containing gases for replenishing carbon for a microalgae culture fluid. By controlling the volume ratio and the circulating speeds of the culture fluid in the open runway pool and the culture fluid in the culturing liquid storage tank, the detention time of microalgae cells in the bright runway pool and the dark culturing liquid storage tank is adjusted, and bright and dark forced circulation of the microalgae cells is realized. By adopting the device and the method, the problems of poor bright and dark mixing of cells, low light energy utilization ratio and difficulties in charging gas and replenishing carbon in the culturing process of the conventional open runway pool are solved, the culturing efficiency, the growing rate and the area yield of microalgae are increased remarkably, and the investment and running costs of a mass microalgae culturing system are reduced.

Description

technical field [0001] The invention belongs to the field of microalgae cultivation, in particular to a device for large-scale cultivation of microalgae. [0002] The invention also relates to a method for large-scale cultivation of microalgae by using the above-mentioned device. Background technique [0003] Microalgae can effectively use light energy, CO 2 and inorganic salts to synthesize proteins, fats, carbohydrates and a variety of high value-added bioactive substances, which can be used to produce health food, food additives, feed and other chemicals through microalgae cultivation. Especially in recent years with the development of bioenergy technology, some oily microalgae can be used in saline-alkali land, desert, Marine aquaculture has the great advantage of not competing for land and people for food, and can use carbon dioxide in factory exhaust gas as a carbon source. Countries all over the world have taken it as a very important way to solve the shortage of bi...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12N1/12C12R1/89
CPCC12M21/02C12M29/06C12M41/00
Inventor 张维尹久盛刘天中陈昱王俊峰陈晓琳彭小伟陈林高莉丽
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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