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Hemoglobin test board and preparation method and application thereof

A technology of hemoglobin and detection board, which is applied in the direction of chemical reaction of materials and material analysis by observing the influence of chemical indicators, etc., to achieve the effect of high degree of automation, scientific design and development and technical route, and stable performance.

Inactive Publication Date: 2012-03-21
苏州百倍生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a hemoglobin detection board and its preparation method and application, which solves the problem of simultaneous detection of hemoglobin concentration in multiple samples, has stable detection results, high detection efficiency, exquisite appearance, and is convenient and easy to use

Method used

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  • Hemoglobin test board and preparation method and application thereof
  • Hemoglobin test board and preparation method and application thereof
  • Hemoglobin test board and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Single serving hemoglobin test panel

[0032] The detection method of the hemoglobin detection board is based on the detection method of azidemethemoglobin to realize the quantitative determination of hemoglobin. The detection result of this method is stable and the accuracy is high. It is one of the internationally recognized hemoglobin detection methods.

[0033] The hemoglobin detection plate includes a sample pretreatment area and a solid-phase reagent reaction area. The whole blood is lysed by a cytolytic agent to lyse hemoglobin in the sample pretreatment area, and then diffused and sent to the solid-phase reagent reaction area, where the solid-phase reaction carrier film in the solid-phase reagent reaction area is pre-cured with the reaction reagent. The reaction reagent reacts with the hemoglobin in it, and finally produces an obvious color change on the carrier film. According to the different concentration of Hb of the analyte, it shows different degrees of co...

Embodiment 2

[0046] Four-person hemoglobin test panel

[0047] The detection principle is the same as above.

[0048] main components

[0049] The solid-phase reagent reaction carrier on each window of the hemoglobin detection plate and the corresponding sample pad contain the following substances:

[0050]

[0051]Each hemoglobin detection plate has 4 windows, that is, the total amount of reagents is:

[0052]

[0053] Preparation of hemoglobin detection plate: (such as figure 2 )

[0054] ① Cutting and fixing: Cut the solid-phase reaction carrier film 1, the sample pad 4, and the sample tank 3 according to the specifications, and process and manufacture the supporting substrate and the supporting cover with matching sizes, in this embodiment, the hard substrate 5 and the plastic strip rack 2. The sample pad 4 is added to the sample loading tank 3; the solid-phase reaction carrier film 1 is heat-sealed and covered on the plastic strip rack 2 at 500°C.

[0055] ②Sampling: Acco...

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PUM

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Abstract

The invention discloses a hemoglobin test board and a preparation method and an application thereof. The hemoglobin test board is characterized by comprising a sample pretreatment are and a solid reagent reaction area, wherein complete blood cells are dissolved and broken in a plurality of sample pretreatment devices of the sample pretreatment area so as to dissolve out hemoglobin and to transmit the hemoglobin to the solid reagent reaction area with the reaction reagent being previously solidified so as to complete the chemical reaction and to obtain the test result. The invention also discloses a preparation method of the hemoglobin test board. Due to the adoption of the hemoglobin test board, the hemoglobin density of multiple people can be simultaneously tested at one time, the reaction is rapid, and the accuracy and the precision are high; and the repeatability is good, and the requirement of large-scale quick test in hospitals and mobile blood collecting vehicles can be satisfied.

Description

technical field [0001] The invention relates to a device for rapidly detecting hemoglobin of multiple people at the same time, belongs to the technical field of medical equipment, and particularly relates to a hemoglobin detection board and its preparation method and application. Background technique [0002] Hemoglobin is a protein responsible for carrying oxygen in the human body. As an important substance in the blood to complete the exchange of oxygen and carbon dioxide, it is the basic condition for maintaining life activities. Hemoglobin detection is the most important indicator for the clinical diagnosis of anemia, and it is also an important indicator for auxiliary diagnosis of other types of blood diseases. At present, the principle of clinical detection of hemoglobin is generally based on cyanide methemoglobin method (HiCN method), azidemethemoglobin method (HiN3 method) or sodium dodecyl sulfate hemoglobin method (SLS-Hb method), in which cyanide methemoglobin Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 吕炜锋郝曦东白伟吕丽媛赵蕊
Owner 苏州百倍生物科技有限责任公司
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