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Serum-free culture fluid for culture of salivary gland epidermal cell and salivary gland stem cell of mammals

A serum-free culture medium and epithelial cell technology, which can be used in cell biology research, mammalian salivary gland epithelial cells and salivary gland stem cell culture fields, and can solve the problems of unclear components and high price.

Active Publication Date: 2014-07-30
INST OF DONGGUAN SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

N2 is a commercial product with unclear composition and expensive

Method used

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  • Serum-free culture fluid for culture of salivary gland epidermal cell and salivary gland stem cell of mammals
  • Serum-free culture fluid for culture of salivary gland epidermal cell and salivary gland stem cell of mammals
  • Serum-free culture fluid for culture of salivary gland epidermal cell and salivary gland stem cell of mammals

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Embodiment 1

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Embodiment 2

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Embodiment 3

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PUM

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Abstract

The invention provides serum-free culture fluid for culture of salivary gland epidermal cells and salivary gland stem cells of mammals, which is prepared by using MCDB153HAA as basic culture medium and adding amino acid, vitamins, salts, lipid, trace elements, buffer fluid, hormone-like compounds, transgenic metalloprotein, antioxidants, seralbumin, glucide, purine, pyrimidine base substances and pH value indicators. The serum-free culture fluid has the advantages that salivary gland epidermal cells of the mammals can vigorously grow, good cell activity and physiological properties are maintained, in addition, the serum-free culture fluid is also suitable for the salivary gland epidermal cell culture, is particularly suitable for the field of study relevant to biological tissue engineering and belongs to the commercial cell culture fluid.

Description

technical field [0001] The invention relates to the field of cell biology research, in particular to the technical field of culture of mammalian salivary gland epithelial cells and salivary gland stem cells. Background technique [0002] Salivary glands are one of the important exocrine glands in mammals, and their secretions can help digestion, protect mucous membranes, and resist bacteria and bacteria. The main function of the salivary glands is to produce and secrete saliva. Under normal circumstances, the amount of saliva secreted by a person is about 1 to 1.5 liters per day. 25% of saliva is secreted by the parotid gland, 5% by the sublingual gland, 10% by various minor salivary glands (lip gland, buccal gland, palatine gland, etc.), and 60% by the submandibular gland. At present, nearly 30 biologically active peptides have been found or isolated from the submandibular glands of animals and humans, such as nerve growth factor (NGF), epidermal growth factor (EGF), endot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071C12N5/074
Inventor 张雁汪华
Owner INST OF DONGGUAN SUN YAT SEN UNIV
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