Liver cancer metastasis prognosis quantitative antibody chip and reagent kit

An antibody chip, a technology for liver cancer metastasis, applied in the fields of biotechnology and medicine

Inactive Publication Date: 2012-06-20
ZHONGSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no specific and sensitive liver cancer metastasis prediction system that can be used for clinical metastasis and recurrence prediction.

Method used

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  • Liver cancer metastasis prognosis quantitative antibody chip and reagent kit
  • Liver cancer metastasis prognosis quantitative antibody chip and reagent kit
  • Liver cancer metastasis prognosis quantitative antibody chip and reagent kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Liver cancer metastasis prediction quantitative antibody chip and its preparation

[0027] A liver cancer metastasis prediction quantitative antibody chip, including a substrate, the substrate contains:

[0028] (1) Mouse anti-human monoclonal antibody of OPN, 0.5mg / ml, (manufacturer R&D, product number MAB14332);

[0029] (2) Mouse anti-human monoclonal antibody of ICAM, 0.7mg / ml, (manufacturer R&D, product number MAB720);

[0030] (3) Mouse anti-human monoclonal antibody to VEGF, 1.0 mg / ml, (manufacturer R&D, product number MAB293);

[0031] (4) MMP9 mouse anti-human monoclonal antibody, 0.2mg / ml, (manufacturer R&D, product number MAB9111);

[0032] (5) Mouse anti-human monoclonal antibody to IL-8, 0.5mg / ml, (manufacturer R&D, product number MAB208);

[0033] (6) E-Selectin mouse anti-human monoclonal antibody, 0.5mg / ml, (manufacturer R&D, product number BBA2);

[0034] (7) Negative control PBS (concentration 0.01mol / l, manufacturer Gibco, product numbe...

Embodiment 2

[0038] Example 2: Liver cancer metastasis prediction kit

[0039] A liver cancer metastasis prediction kit, comprising:

[0040] (1) The quantitative antibody chip described in Example 1;

[0041] (2) Antigen standards containing OPN, ICAM, VEGF, MMP9, IL-8 and E-Selectin (the stock solution concentration is 0.5mg / ml, dissolved in 0.01M PBS);

[0042] (3) Biotinylated monoclonal detection antibody for OPN [Catalog No. BAF1433], biotinylated monoclonal detection antibody for ICAM [Cat. No. BAF720], biotinylated monoclonal detection antibody for VEGF [Catalog No. BAF293], MMP9 Biotinylated Monoclonal Detection Antibody [Cat. No. BAF911], IL-8 Biotinylated Monoclonal Detection Antibody [Cat. No. BAF208] and E-Selectin Biotinylated Monoclonal Detection Antibody [Cat. R&D companies;

[0043] (4) Blood sample collection device

[0044] (5) Operating instructions.

Embodiment 3

[0045] Example 3: The method of using the Liver Cancer Metastasis Prediction Kit

[0046] 1. Reagents used:

[0047] (1) The liver cancer metastasis prediction kit described in Example 2;

[0048] (2) 1×Gentel blocking solution, produced by GenTel Biosurfaces in the United States, the product number is (2-1014);

[0049] (3) 1×Gentel wash cleaning solution, the product number produced by GenTel Biosurfaces in the United States is (2-1016);

[0050] (4) Cy3-labeled avidin containing 10% goat serum, produced by Invitrogen in the United States, the product number is (43-4315);

[0051] (5) 1×rinse solution, produced by GenTel Biosurfaces in the United States, the product number is (2-1018);

[0052] 2. The equipment used:

[0053] (1) SIMplex device (Whatman Schleicher & Schell, UK);

[0054] (2) Jingxin? LuxScan? 10K microarray and high-resolution laser confocal chip scanner for image data processing produced by Beijing Boao Biological Co., Ltd.;

[0055] 3. Specific opera...

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Abstract

The invention provides a liver cancer metastasis prognosis quantitative antibody chip and a reagent kit. The quantitative prognosis chip comprises a substrate, specific antibodies, positive control and negative control. The specific antibodies of cancer related proteins are dispensed on the substrate. The liver cancer metastasis prognosis quantitative antibody is characterized in that the cancer related proteins include OPN (osteopontin), ICAM (intercellular cell adhesion moleculars), VEGF (vascular endothelial growth factors), MMP (matrix metal proteinase) 9, IL (interleukin) -8 and E-selectin. The reagent kit consists of the quantitative antibody chip adopting the OPN, the ICAM, the VEGF, the MMP9, the IL-8 and the E-selectin as detection objects, an antigen protein calibrator comprising OPN, ICAM, VEGF, MMP9, IL-8 and E-selectin and a biotinylated monoclonal detecting antibody comprising OPN, ICAM, VEGF, MMP9, IL-8 and E-selectin. The liver cancer metastasis prognosis quantitative antibody chip is successfully created, prognosis accuracy achieves 83% when the liver cancer metastasis prognosis quantitative antibody chip is applied to prognosis of liver cancer metastasis, and the liver cancer metastasis prognosis quantitative antibody chip and the reagent kit have an important clinical significance for judging prognosis of liver cancer patients.

Description

technical field [0001] The invention relates to a liver cancer metastasis prediction quantitative antibody chip and a kit, which belong to the fields of biotechnology and medical technology. Background technique [0002] Liver cancer is a malignant tumor with a very high degree of malignancy and a very poor prognosis. About half of the world's liver cancer patients are concentrated in China, and its fatality rate ranks second among all tumors in my country. Liver cancer is the second leading cause of cancer death in my country, and more than 50% of primary liver cancers in the world occur in my country. The high postoperative recurrence rate of metastasis (5-year recurrence rate reaches 70%, small liver cancer also reaches 50%) has become a bottleneck to further improve long-term efficacy, and is also an important key to conquer liver cancer. [0003] In recent years, with the in-depth research in basic medical fields such as immunology, molecular biology and biochemistry,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
Inventor 郭坤刘银坤康晓楠高冬梅孙淳
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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