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Complete method for identifying and screening heat-resistant bottle gourd stocks

A gourd and heat-resistant technology, which is applied in the field of heat-resistant identification of gourd-type rootstock materials, can solve problems such as unsatisfactory, low accuracy, and environmental impact, and achieve the effects of short time consumption, low cost, and accurate results

Inactive Publication Date: 2012-08-01
NINGBO ACAD OF AGRI SCI
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Problems solved by technology

At present, the heat resistance identification of gourd rootstocks mostly uses the field natural identification method, which is easily affected by the environment, has low accuracy, takes a long time, and cannot meet the requirements for rapid screening of target materials in the process of variety selection.

Method used

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  • Complete method for identifying and screening heat-resistant bottle gourd stocks

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Embodiment Construction

[0007] The specific implementation of the present invention will be described by taking the heat-resistant identification and screening of heat-resistant materials of 37 gourds as an example.

[0008] (1) Germination of gourd seeds at high temperature

[0009] Select 37 gourd materials with 90 plump and pest-free normal seeds, firstly scald them in water at 55-60°C for 20 minutes, and then soak the seeds in warm water at 30°C for 12 hours. After soaking the seeds, take out the seeds and drain the water, evenly place them in a petri dish (PL-9) covered with four layers of gauze, the germination density is 15 grains / petri dish, and moisturize with deionized water. Divide into two groups (45 capsules / group, 3 Petri dishes / group), and germinate in the dark under the conditions of 30-35°C and 40-45°C respectively. According to the 1996 edition of the International Seed Inspection Regulations, the germination standard is when the seed germ is half as long as the seed. Rinse the se...

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Abstract

The invention relates to a complete method for identifying and screening heat-resistant bottle gourd stocks, which belongs to the field of biology. Rapid screening of heat-resistant materials for variety breeding is based on establishment of a reliable heat resistance identification method. The natural field identification method is susceptible to environment and unable to accurately screen target materials. Therefore, inventing a complete method for rapidly and accurately identifying bottle gourd stock heat resistance by artificial stressing and statistical means is urgent. According to the technical scheme, the complete method includes: using bottle gourd high-temperature seeds to sprout and testing electric conductivity in the seedling stage; calculating identification indexes, namely relative germination rate (40-45 DEG C / 30-35 DEG C), relative germination index (40-45 DEG C / 30-35 DEG C) and relative electric conductivity (6h / 0h); and identifying and screening the bottle gourd seeds by the clustering function of software SPSS13.0. The complete method is simpler in operation flow, consumes less time, is lower in cost, and can be used for identifying heat resistance of the bottle gourd stock material more quickly, more accurately and more conveniently.

Description

1. Technical field [0001] The invention belongs to the field of biology, and in particular relates to a method for identifying heat resistance of gourd-type rootstock materials. 2. Background technology [0002] Gourd (Bottle gourd, Lagenaria siceraria (Molina) Standl.), also known as gourd, flat cattail, gourd, belongs to Cucurbitaceae Cucurbita, an annual climbing herb. It has a well-developed root system, and as a grafting rootstock for important melon crops such as cucumbers, watermelons and melons, it can effectively improve the adaptability of plants to adverse environments. In most areas of my country, the temperature in open fields in summer is above 35°C, while that in protected areas is above 40°C. High temperature stress is the main factor restricting summer cultivation and annual supply of melon crops. Breeding gourd rootstock varieties with strong heat tolerance and improving the heat tolerance of melon crops through grafting is an effective way to solve the ab...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01G7/00
Inventor 王毓洪宋慧应泉盛王迎儿张香琴严蕾艳张华峰
Owner NINGBO ACAD OF AGRI SCI
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