Pepper CaCOI1.2 gene, recombinant expression vector and application thereof

Abstract

The invention discloses a pepper CaCOI1.2 gene. An open reading frame sequence is composed of the 186th to 1997th nucleotide of the SEQ (sequence) ID NO.1. The invention further discloses a recombinant expression vector containing the pepper CaCOI1.2 gene, a transformant, an application of the pepper CaCOI1.2 gene to large-fruit pepper plant breeding and a specific application method. Research results show that an over-expression vector containing the pepper CaCOI1.2 gene is introduced into a pepper of new Su Jiao Wang strain through agrobacterium mediates, and pepper fruits of the obtained transgenic positive plants are bigger than those of non-transgenic pepper plants, so that the yield of the pepper is greatly increased; the CaCOI1.2 gene is a self gene of the pepper, thereby the transgenic plants obtained by introducing the CaCOI1.2 gene into pepper plants have no influence on the environment safety and food safety; and the pepper transgenic method is high in transduction efficiency.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and relates to a gene, a recombinant expression vector and a transformant of the gene, as well as the application of the gene and a specific application method. Background technique [0002] Pepper is an important crop of Solanaceae, which is widely cultivated all over the world. Chili is also an important condiment, usually used in kimchi, barbecue and other foods together with other condiments. Pepper has high nutritional value and is rich in vitamins A, B, C, D, E and various mineral elements such as molybdenum, manganese, potassium, etc. Capsaicin extracted from peppers also has medicinal value, and has a certain therapeutic effect on lumbar pain, neuralgia, rheumatic pain and allergic rhinitis. [0003] Although traditional plant breeding has made great contributions in the process of improving crops (such as quality traits), conventional breeding is difficult and time-consumin...

AI Technical Summary

Problems solved by technology

However, at present, there are relatively many reports on the use of transgenic technology to increase the resistance to diseases and insect pests, herbicides and salt-alkali resistance of peppers, but there are few reports on the increase of pepper production.

In addition, due to the low and unstable regeneration rate and low transformation efficiency of pepper compared with other Solanaceae plants, there is no universally suitable transformation model for most pepper varieties and different explants, and the use of transgenes has also increased accordingly. Difficulty in technology-oriented improvement of pepper traits

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