Method for preparing angiotensin converting enzyme inhibitory peptide from shrimp shell

An angiotensin-converting enzyme technology, which is applied in the field of angiotensin-converting enzyme (ACE) inhibitory peptides, can solve environmental pollution and other problems, and achieve the effect of easy-to-obtain and abundant raw materials and increase added value

Inactive Publication Date: 2012-09-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] The invention provides a method for preparing angiotensin-converting enzyme inhibitory peptides from shrimp shells, which solves the problem of environmental pollution caused by shrimp shells, a by-product of shrimp processi

Method used

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  • Method for preparing angiotensin converting enzyme inhibitory peptide from shrimp shell
  • Method for preparing angiotensin converting enzyme inhibitory peptide from shrimp shell
  • Method for preparing angiotensin converting enzyme inhibitory peptide from shrimp shell

Examples

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Embodiment 1

[0027] A preparation method of shrimp shell angiotensin-converting enzyme (ACE) inhibitory peptide, adopting the following steps:

[0028] A) Clean the shrimp shells and remove impurities, bake at 40°C for 24 hours, grind the dried samples into powder with a tissue grinder, pass the powder through a 40-mesh sieve to make shrimp shell powder, and then add water to prepare a mass fraction of 5%. Shrimp shell powder solution;

[0029] B) Adjust the pH of the above shrimp shell powder solution to 6.5, then add neutral protease 5000U / g shrimp shell powder solution, put it in a water bath at 45°C for 4 hours, and carry out the reaction under stirring, and keep the temperature and pH constant , to obtain shrimp shell protein hydrolyzate;

[0030] C) Inactivate the above shrimp shell protein hydrolyzate in a 95°C water bath for 15 minutes, take it out and cool it to room temperature, centrifuge it at 3000r / min for 10 minutes, take the supernatant, and perform ultrafiltration with an ...

Embodiment 2

[0033] A preparation method of shrimp shell angiotensin-converting enzyme (ACE) inhibitory peptide, adopting the following steps:

[0034] A) Clean the shrimp shells and remove impurities, dry them at 60°C for 24 hours, grind the dried samples into powder with a tissue grinder, pass the powder through a 100-mesh sieve to make shrimp shell powder, and then add water to prepare a mass fraction of 1 % shrimp shell powder solution;

[0035] B) Adjust the pH of the above shrimp shell powder solution to 7.5, then add neutral protease 1000U / g shrimp shell powder solution, put it in a 55°C water bath for 1 hour, and carry out the reaction under stirring, and keep the temperature and pH constant , to obtain shrimp shell protein hydrolyzate;

[0036] C) Inactivate the above shrimp shell protein hydrolyzate in a water bath at 95°C for 15 minutes, take it out and cool it to room temperature, centrifuge at 5000r / min for 20 minutes, take the supernatant, and perform ultrafiltration with an...

Embodiment 3

[0039] A preparation method of shrimp shell angiotensin-converting enzyme (ACE) inhibitory peptide, adopting the following steps:

[0040]A) Clean the shrimp shells and remove impurities, dry them at 50°C for 24 hours, grind the dried samples into powder with a tissue grinder, pass the powder through a 60-mesh sieve to make shrimp shell powder, and then add water to prepare a mass fraction of 2.5 % shrimp shell powder solution;

[0041] B) Adjust the pH of the above shrimp shell powder solution to 7, then add neutral protease 2000U / g shrimp shell powder solution, put it in a water bath at 50°C for 2 hours, and carry out the reaction under stirring, and keep the temperature and pH constant , to obtain shrimp shell protein hydrolyzate,;

[0042] C) Inactivate the above-mentioned shrimp shell protein hydrolyzate in a 95°C water bath for 15 minutes, take it out and cool it to room temperature, centrifuge it at 4000r / min for 15 minutes, take the supernatant, and use an ultrafiltra...

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Abstract

The invention discloses a method for preparing angiotensin converting enzyme (ACE) inhibitory peptide from shrimp shell. The method comprises the following steps: a, cleaning and drying shrimps and grinding and sieving and adding water to prepare 1-5 percent shrimp shell powder solution; b, adjusting the pH value, adding neutral protease and carrying out enzymatic hydrolysis at constant temperature; c, inactivating enzyme, separating and purifying; and d, concentrating, freezing and drying to obtain the ACE inhibitory peptide of the shrimp shell. The ACE inhibitory peptide obtained by the method is lower in molecular weight and stable in activity and can quickly enter blood for circulating and is absorbed and utilized by a human body; the hypotensive effect on people with normal blood pressure is avoided or the excessive hypotensive reduction is also prevented; and the ACE inhibitory peptide is obtained by adopting food-grade enzyme under mild conditions and has high safety and no toxic or side effect. The method is simple in operation; and the shrimp shell which is low price, abundant and easily obtained is used as raw materials, and thus the added value of shrimp products is increased and the pollution to the environment is avoided.

Description

technical field [0001] The invention relates to an angiotensin-converting enzyme (ACE) inhibitory peptide, in particular to a method for preparing angiotensin-converting enzyme (ACE) inhibitory peptides from shrimp shells by enzymolysis reaction using shrimp shells as raw materials. Background technique [0002] Hypertension is a disease that is extremely harmful to human health. It can induce a series of cardiovascular and cerebrovascular diseases and lead to human death. According to the "Guidelines for the Prevention and Treatment of Hypertension in China 2010 Revised Edition" promulgated by the National Center for Cardiovascular Diseases, it is estimated that the prevalence of hypertension in my country is on the rise, with 1 out of every 5 adults suffering from hypertension. 200000000. Controlling hypertension has become an urgent task in our country's medical and health services. [0003] Angiotensin-converting enzyme (ACE) is ubiquitous in mammalian tissues and is a ...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
Inventor 罗自生王延圣施旭丹
Owner ZHEJIANG UNIV
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