Technology for quickly detecting tabersonine in voacanga seeds

Abstract

The invention discloses a technological method for detecting tabersonine in voacanga seeds. The technological method includes (1) sampling and crushing; uniformly sampling from various points, uniformly mixing voacanga seed samples and crushing the voacanga seed samples for standby application; (2) extracting: adding 3g of the crushed voacanga seed samples into an extracting tank, further adding 25ml of methanol in the extracting tank, extracting by the aid of ultrasonic waves for 30 minutes, allowing the mixture to stand for 30 minutes and cooling the mixture to reach the room temperature; and (3) detecting liquid phases: taking from 1ml to 10ml of supernate obtained in the step (2) into a measuring flask, metering the supernate to a certain graduation, using the supernate as to-be-tested liquid, respectively precisely metering 5 microliters of comparison liquid with the concentration of 1mg / ml and 5 microliters of the to-be-tested liquid and filling the comparison liquid and the to-be-tested liquid into a liquid chromatograph, and recording the peak area and obtaining the content of the to-be-tested liquid according to a content computational formula. The technological method has the advantages that consumption of raw materials is low, required time is short, accuracy of detection results is high, and relative errors are fewer.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to a detection technology for tabonin, in particular to a process method for detecting tabonin from potato seeds. Background technique [0002] Potato fruit (Voacanga) is an African plant, and an alkaloid can be isolated from its seeds, called tabersonine, also known as willow licorice. Studies have shown that it has lowering blood pressure and anti-tumor effects, and it is also used for depression, anxiety, and emotional instability caused by stroke sequelae, ischemic hypertensive encephalopathy, and cerebrovascular diseases, and is suitable for the elimination of symptoms of premature degeneration , such as dizziness, headache, memory loss, inattention, aphasia, Meniere's syndrome, etc., can also be used for retinal hemorrhage, nervous tachycardia and other autonomic dysfunction. [0003] In the prior art, the heating and reflux extraction technology is generally used to detect the content...

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the heating and reflux extraction technology is generally used to detect the content of tabonin in the potato, including several steps such as sampling and crushing of the potato, extraction, concentration, crystallization, suction filtration, and drying. The amount of raw materials used in the sampling stage of this method is Usually about 100g is needed, the amount of raw materials is large, there are many steps, and the detection time is too long, which is not conducive to the rapid detection requirements of potato taraponin under specific circumstances.

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