Method for rapidly obtaining loose calluses of grapes and for long-term succeeding maintenance of grapes

Abstract

The invention provides a method for rapidly obtaining loose calluses of grapes and for long-term succeeding maintenance of the grapes. The method sequentially comprises the following steps of: preparing sterile strains of the grapes; carrying out startup culture on the strains; preparing the loose calluses of the grapes to obtain the loose calluses of the grapes; grafting the obtained loose calluses of the grapes to a first culture medium to realize the succeeding maintenance of the grapes for at least two generations; and transferring the loose calluses of the grapes to a second culture medium to realize the succeeding maintenance of the grapes for one generation. The long-term succeeding maintenance of the loose calluses of the grapes can be implemented by repeatedly and alternately carrying out the steps, and the first culture medium and the second culture medium used in the succeeding maintenance process are both placed in a culturing room. The method disclosed by the invention has the advantages that the obtained loose calluses of the grapes have the characteristics of high induction efficiency and high quality and can be subjected to long-term succeeding maintenance, thereby laying a good foundation for researches on the production of secondary metabolites through grape cell culturing, biological technology researches and the like.

Description

【Technical field】 [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for rapidly obtaining grape loose callus and its long-term subculture maintenance. 【Background technique】 [0002] Grape (Vitis vinifera L.), a deciduous vine of the genus Vitis, accounts for almost a quarter of the world's fruit production. It has high nutritional value and can be made into grape juice, raisins and wine. In recent years, the scientific discovery of the "French Paradox" has drawn widespread attention to the bioactive substances in grapes. Studies have shown that grapes are rich in phenolic and phenolic bioactive substances such as resveratrol, catechin, epicatechin and ellagic acid, which have important pharmacological functions and biological activities, and have good health effects, especially It is resveratrol that has anti-cancer activity and is favored by the market. [0003] With the continuous expansion of market demand, the origi...

AI Technical Summary

Problems solved by technology

In order to solve these problems, the most critical technology at present is to establish a method for quickly obtaining grape loose callus and its long-term subculture maintenance. The low efficiency of callus induction, the poor quality of formed callus, and the inability to maintain long-term subcultures lead to slow research on the production of secondary metabolites in grape cell culture.

Up to now, some progress has been made in callus induction using grape leaves, petioles, stems, etc. There are few reports on the long-term subculture maintenance of the loose callus and the loose callus

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