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Application of brucella flagellin bmeii1112 in preparation of brucella subunit vaccine

A BMEII1112, subunit vaccine technology, applied in the preparation methods of peptides, antibacterial drugs, bacterial antigen components, etc., can solve the problems such as the immune protection of BMEII1112 that have not yet been seen

Inactive Publication Date: 2015-07-29
INST OF PLA FOR DISEASE CONTROL & PREVENTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the immune protection of BMEII1112

Method used

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  • Application of brucella flagellin bmeii1112 in preparation of brucella subunit vaccine
  • Application of brucella flagellin bmeii1112 in preparation of brucella subunit vaccine
  • Application of brucella flagellin bmeii1112 in preparation of brucella subunit vaccine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Embodiment 1, recombinant expression Brucella flagellin BMEII1112

[0066] Utilize the Gateway technology to make the brucella flagellin BMEII1112 gene obtain expression in escherichia coli, and specific method comprises the following steps:

[0067] 1. PCR amplification of the flagellin BMEII1112 gene of Brucella

[0068] 1. Primer design

[0069] Primer 5.0 software was used to design primers for amplifying the Brucella flagellin BMEII1112 gene and the BMEI0748 (L7 / L12, known Brucella protective antigen) ORF gene as a positive control, and at the 5' of each pair of primers At the same time, a pair of general primers AttB1 and AttB2 were designed. The primer sequences are shown in Table 1, provided by Beijing Aoke Biological Co., Ltd. synthesis.

[0070] Table 1 Primer Sequence

[0071]

[0072] Note: AttB1 and AttB2 are general sequences.

[0073] 2. PCR amplification

[0074] Amplify the BMEII1112 gene and the BMEI0748 gene as a positive control in two steps...

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Abstract

The invention discloses a novel application of brucella flagellin BME II 1112 in preparation of brucella subunit vaccines. The experimental results show that a BME II 1112 recombinant protein and a freund's adjuvant are subjected to combined immunization to be induced so as to produce a specific antibody, stimulate the cell immunity, and generate a better immune protecting effect for the brucella, thereby being capable of being utilized to prepare the brucella subunit vaccines. The application of brucella flagellin BME II 1112 in preparation of brucella subunit vaccines plays an important role in the epidemic prevention of the brucella, so that the application prospect is wide.

Description

technical field [0001] The invention relates to a new application of proteins, in particular to the application of a Brucella flagellin BMEII1112 in preparing Brucella subunit vaccines. Background technique [0002] Brucellosis (referred to as brucellosis) caused by Brucella is a typical zoonotic infectious disease, which has caused huge economic losses to my country's animal husbandry production and seriously threatened people's healthy life. Brucellosis is a chronic infectious disease, prevention of the disease is an important measure to control the disease. Currently, prevention of brucellosis is mainly achieved through vaccination. Vaccines prepared with inactivated and attenuated Brucella pathogens have received effective immune responses and immune protection after immunizing animals, but there are strong allergic reactions after vaccination, difficulty in differential diagnosis between immunity and infection, and bacterial infection in individual cases. It is diffic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/10A61P31/04C12N15/63C12N15/70C07K14/23C07K1/22C12R1/01
Inventor 王玉飞陈泽良黄留玉袁静宋宏彬杜昕颖汪舟佳柯跃华
Owner INST OF PLA FOR DISEASE CONTROL & PREVENTION
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