Tumor suppressor gene NDRG2 based application for improving breast cancer chemotherapy sensibility

A tumor suppressor gene, breast cancer technology, applied in the direction of gene therapy, genetic material components, drug combination, etc., can solve the problems affecting the clinical application effect of doxorubicin, and achieve the effect of improving the sensitivity of breast cancer chemotherapy

Inactive Publication Date: 2012-11-21
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

These toxic and side effects have seriously affected the clinical application effect of doxorubicin

Method used

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  • Tumor suppressor gene NDRG2 based application for improving breast cancer chemotherapy sensibility
  • Tumor suppressor gene NDRG2 based application for improving breast cancer chemotherapy sensibility
  • Tumor suppressor gene NDRG2 based application for improving breast cancer chemotherapy sensibility

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Embodiment Construction

[0049] The present invention will be further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations.

[0050] 1. Construction of NDRG2 gene expression vector and virus packaging

[0051] The expression vector of NDRG2 was constructed based on the Gateway system pLenti6.3 / V5-DEST vector of Invitrogen Company. First, use molecular cloning technology to clone NDRG2 into the entry vector pENTR-3C (cloning through BamHI and EcoRI sites), and then use LR recombinase to recombine NDRG2 into the pLenti6.3 / V5-DEST vector to obtain the Plenti6.3-NDRG2 vector , the NDRG2 gene sequence in the vector was verified by DNA sequencing.

[0052] 2. Establish a stable transfected cell line with high expression of NDRG2

[0053]Breast cancer cells MCF-7 and MDA-MB-231 were respectively selected as target cells, and the parental cells were treated with the drug Blasticidin S (blasticidin) respectively, and the a...

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Abstract

The invention discloses a tumor suppressor gene NDRG2 based application for improving breast cancer chemotherapy sensibility. The tumor suppressor gene NDRG2 is transfected into a breast cancer cell through an expression vector or a virus vector, and the result shows that the tumor suppressor gene NDRG2 can improve the breast cancer chemotherapy sensibility and can be applied to preparation of relevant drugs. The NDRG2 prompts a deoxyribonucleic acid (DNA) damage ability of Adr induced p53 wild type breast cancer cells, improves morphology damage of Adr to p53 wild type breast cancer cells, improves a proliferation inhibiting ability of the Adr to the p53 wild type breast cancer cells, improves the breast cancer chemotherapy sensibility which is independent of specific chemotherapy drugs and further prompts apoptosis of the Adr induced p53 wild type breast cancer cells.

Description

technical field [0001] The invention belongs to the technical field of tumor treatment, and relates to genes related to improving chemotherapy sensitivity, in particular to the application of improving chemotherapy sensitivity of breast cancer based on tumor suppressor gene NDRG2. Background technique [0002] Breast cancer is one of the most common tumors in women. Its incidence rate is the first among women suffering from tumors, accounting for about 1 / 4 of all cancers in women, and it seriously threatens women's health. The treatment of breast cancer is mainly based on surgery combined with chemotherapy and radiotherapy. At present, chemical drugs are still a common method for the treatment of breast cancer and play an important role in the treatment of breast cancer. However, there are many problems in chemotherapy, such as indistinguishable from friend and foe, large side effects, and common drug resistance, which seriously restrict its clinical efficacy. [0003] Dox...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61P35/00
Inventor 张健药立波张永平张璟陈苏宁
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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