Tumor suppressor gene NDRG2 based application for improving breast cancer chemotherapy sensibility
A tumor suppressor gene, breast cancer technology, applied in the direction of gene therapy, genetic material components, drug combination, etc., can solve the problems affecting the clinical application effect of doxorubicin, and achieve the effect of improving the sensitivity of breast cancer chemotherapy
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[0049] The present invention will be further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations.
[0050] 1. Construction of NDRG2 gene expression vector and virus packaging
[0051] The expression vector of NDRG2 was constructed based on the Gateway system pLenti6.3 / V5-DEST vector of Invitrogen Company. First, use molecular cloning technology to clone NDRG2 into the entry vector pENTR-3C (cloning through BamHI and EcoRI sites), and then use LR recombinase to recombine NDRG2 into the pLenti6.3 / V5-DEST vector to obtain the Plenti6.3-NDRG2 vector , the NDRG2 gene sequence in the vector was verified by DNA sequencing.
[0052] 2. Establish a stable transfected cell line with high expression of NDRG2
[0053]Breast cancer cells MCF-7 and MDA-MB-231 were respectively selected as target cells, and the parental cells were treated with the drug Blasticidin S (blasticidin) respectively, and the a...
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