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Photinia serrulata tissue culture seedling rooting culture medium and culture method for photinia serrulata tissue culture seedling rooting

A rooting medium and rooting culture technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of vitrification, low root activity, and few rooting numbers, and control callus and vitrification. , the effect of robust growth

Inactive Publication Date: 2012-12-26
CHONGQING UNIV OF ARTS & SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problems of Photinia fragrans tissue culture and industrial planting have not been completely resolved. When MS medium is used for rooting and culturing of Photinia fragrans tissue culture seedlings, the following problems often occur: the callus at the base of the rooted plantlets in tissue culture is enlarged, the number of roots is small, and the rooting rate is low , low root activity, prone to callus and vitrification after multiple subcultures

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  • Photinia serrulata tissue culture seedling rooting culture medium and culture method for photinia serrulata tissue culture seedling rooting
  • Photinia serrulata tissue culture seedling rooting culture medium and culture method for photinia serrulata tissue culture seedling rooting
  • Photinia serrulata tissue culture seedling rooting culture medium and culture method for photinia serrulata tissue culture seedling rooting

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Embodiment Construction

[0014] The present invention will be described in detail below in conjunction with specific embodiments.

[0015] 1. Experimental materials

[0016] The tissue-cultured subcultured seedlings of Photinia fragrans in the tissue culture room of the Flower Research Institute of Chongqing University of Arts and Sciences were used as experimental materials.

[0017] 2. Experimental method

[0018] Determine the basic formula (see Table 1) of Photinia fragrans rooting medium according to preliminary experimental results and long-term practical experience, and KNO in the basic formula 3 , NH 4 NO 3 , NAA, IBA and other 4 components were adjusted respectively, while other components and their concentrations remained unchanged (see Table 2). Observe the rooting and growth of Photinia fragrans tissue-cultured subculture seedlings under different concentrations of each component, in order to Optimizing the formulation of rooting medium. where KNO 3 and NH 4 NO 3 It is mainly aimed...

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Abstract

The invention discloses a photinia serrulata tissue culture seedling rooting culture medium and a culture method for the photinia serrulata tissue culture seedling rooting. The photinia serrulata tissue culture seedling rooting culture medium provided by the invention is characterized in that on the basis of an MS (Murashige and Skoog) culture medium, the types and the concentration of major elements are regulated, the hormone regulation and control, the agar concentration and the like are optimized, the balanced and reasonable nutrition requirements are provided for the photinia serrulata tissue culture rooting seedlings, the fast, efficient and healthy growth of the photinia serrulata tissue culture rooting seedlings are ensured, and the generation of callus and vitrification phenomena is effectively controlled.

Description

technical field [0001] The invention relates to a rooting medium for plant tissue culture, in particular to a rooting medium for tissue culture seedlings of Photinia fragrans and a cultivation method thereof. Background technique [0002] Photinia frasery Dress (Photinia frasery Dress) is a hybrid species of Photinia in the Rosaceae family. It is an evergreen small tree or multi-branched shrub. , has been widely used in gardens. [0003] Because Photinia fragrans is a hybrid species, large-scale sexual reproduction is greatly restricted, and the quality of seedlings is poor and uneven, so it is difficult to meet the huge demand of the market. The propagation methods of Photinia fragrans include cutting propagation, tissue culture propagation, grafting propagation, and sowing propagation, and tissue culture and cuttings are the main propagation methods. Compared with cutting seedlings, tissue culture seedlings are not restricted by seasons, can better maintain the excellent...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 唐建民刘奕清陈泽雄廖林正黄科黄登艳袁海英
Owner CHONGQING UNIV OF ARTS & SCI
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