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A kind of tissue culture rapid seedling raising method of apple rootstock m9

A technology of apple and tissue culture, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of low multiplication coefficient, slow elongation and growth of apples, etc., achieve balanced and reasonable nutritional requirements, increase rooting coefficient, and promote festival growth. elongation effect

Active Publication Date: 2017-03-08
芜湖东源新农村开发股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for fast seedling raising by tissue culture of apple rootstock M9, which solves the problems of slow elongation growth and low multiplication coefficient in the tissue culture stage.

Method used

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Embodiment

[0019] A method for rapidly growing seedlings by tissue culture of apple rootstock M9, characterized in that it comprises the following steps:

[0020] (1) Obtain sterile materials:

[0021] Cut the newly germinated apple buds in spring, soak them in 75% ethanol for 20-30s to clean the material, soak them in pure water for 15-20 minutes, put them on a super-clean workbench, and then use absorbent paper to absorb the buds to dry surface moisture;

[0022] (2) Primary cultivation of apples:

[0023] Take the base of the budlet, cut it into 1cm-long segments, and inoculate it on the budlet induction culture. The composition of the budlet induction medium is: MN+2.0 mg / L 6-BA+0.5 mg / L 2,4- D + 0.2 mg / L NAA+ 30 g / L sugar + 6g / L agar powder, the buds were inoculated in the bud induction medium for 19 days, and subcultured;

[0024] (3) Proliferation and cultivation of apples:

[0025] The buds with callus are inoculated on the adventitious bud proliferation medium with a pH valu...

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PUM

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Abstract

The invention discloses a tissue culture fast breeding method of apple rootstock M9. The tissue culture fast breeding method of the apple rootstock M9 is characterized by comprising the following steps of (1) acquiring a non-bacteria material; (2) initially culturing apples; (3) enrichment culturing the apples; (4) rooting and hardening-off culturing; (5) acclimatizing and transplanting. The tissue culture fast breeding method of the apple rootstock M9 has the beneficial effects that the method is adopted on the basis of an M9 culture medium, the types and the concentrations of major elements are adjusted, and hormone is optimized to adjust and control the agar concentration and the like, so that the balanced and reasonable nutritional requirement is provided for tissue culture seedling subculture multiplication of the apples, the rooting coefficient is improved, and the internode elongation is effectively promoted.

Description

technical field [0001] The invention relates to a method for rapidly growing seedlings by tissue culture of apple rootstock M9. Background technique [0002] Apple is a deciduous tree with oval, serrated leaves and white flowers with a blush. The fruit is round and sweet or slightly sour. It is a common fruit, rich in nutrients, and has the functions of dietotherapy and auxiliary treatment. It is one of the four major fruits in the world. Apples are native to Europe, Central Asia, West Asia and Turkey, and were introduced to China in the nineteenth century. China is the world's largest apple producer, and it is cultivated in Northeast China, North China, East China, Northwest China, Sichuan, and Yunnan. Apple is rich in vitamin nutrition and is the crown of the world's four major fruits. [0003] Traditional apple seedlings are usually propagated by cutting, grafting, branching, layering, etc. These methods may carry one or more viruses or virus-like viruses, which will h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 汪锡文
Owner 芜湖东源新农村开发股份有限公司
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