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Culture medium for subculture multiplication of tissue cultured seedlings of red-flesh kiwifruits

A proliferation medium and subculture proliferation technology, which is applied in the field of subculture proliferation medium for red meat kiwifruit tissue culture seedlings, can solve the problems of weak growth of tissue culture seedlings, slow growth of internode elongation, and low proliferation coefficient, reaching Robust growth, promotion of internode elongation, and effect of increasing proliferation coefficient

Inactive Publication Date: 2014-01-15
CHONGQING UNIV OF ARTS & SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problems of red-flesh kiwifruit tissue culture industrial seedlings have not been completely resolved. When using MS medium for red-flesh kiwifruit tissue culture seedlings to be subcultured and cultured, the following problems often occur: the callus at the base of the rooted rooted seedlings of the tissue culture is enlarged, and the tissue culture seedlings Weak growth potential, large leaves, short stems, slow internode elongation, low multiplication coefficient, etc.

Method used

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  • Culture medium for subculture multiplication of tissue cultured seedlings of red-flesh kiwifruits
  • Culture medium for subculture multiplication of tissue cultured seedlings of red-flesh kiwifruits
  • Culture medium for subculture multiplication of tissue cultured seedlings of red-flesh kiwifruits

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Embodiment Construction

[0017] In order to make the object, technical solution and advantages of the present invention clearer, preferred embodiments of the present invention are described in detail below.

[0018] In the present invention, the tissue-cultured subculture seedlings obtained from the in vitro bud organ induction and multiplication of fine single-plant red-fleshed kiwifruit are used as test materials, and NaH is added on the basis of MS medium. 2 PO 4 2H 2 O and Ca(NO 3 ) 2 4H 2 O, remove CaCl2 2H 2 O, adjusted the concentration of inositol and thiamine hydrochloride, and optimized hormone regulation and agar concentration, etc.

[0019] 1. Optimization of basic medium for subculture and proliferation of red meat kiwifruit tissue culture seedlings

[0020] (1) Materials and methods

[0021] The tissue-cultured subcultured seedlings obtained from the induction culture of fine single-plant isolated bud organs of red-fleshed kiwifruit were transferred to different medium formulas fo...

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Abstract

The invention discloses a culture medium for subculture multiplication of tissue cultured seedlings of red-flesh kiwifruits. On the basis of MS culture medium, through adjusting the types and concentrations of major elements and optimizing the concentration of hormone regulation agar and the like, balanced and reasonable nutritional requirement is provided to the subculture multiplication of the tissue cultured seedlings of the red-flesh kiwifruits, the rapid, efficient and strong growth of the red-flesh kiwifruits is ensured, the inter-node growth is effectively promoted and the multiplication coefficient is improved.

Description

technical field [0001] The invention relates to a rooting medium for plant tissue culture, in particular to a culture medium for red-flesh kiwifruit tissue culture seedling subculture proliferation. Background technique [0002] Kiwi fruit is a kind of woody vine with high economic value. It has thick flesh and juicy, moderately sweet and sour, and rich nutrition. It has become a treasure in the current fruit because of its high nutritional value of vitamin C. It has become a new emerging fruit in the world today. Cultivate fruit trees. The red-fleshed kiwifruit is a characteristic variety in the kiwifruit family. The fruit ovary is bright red, the flesh is yellow-green, the fruit is medium-large, cylindrical, with high uniformity and good commodity performance. . High sugar content, total sugar 13.45%, total acid 0.49%, soluble solids 19.6% to 24%, vitamin C content above 135.77 mg per 100 g, has a very high market prospect and development and utilization value. [0003]...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 唐建民刘奕清陈泽雄廖林正黄科黄登艳袁海英
Owner CHONGQING UNIV OF ARTS & SCI
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