Novel uses of camellia oleifera cake polysaccharide

A technology of camellia cake and use, which is applied in the new application field of camellia cake polysaccharide, and can solve the problems of unclear molecular structure and function, and large-scale industry of camellia polysaccharide.

Inactive Publication Date: 2013-01-23
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because the molecular structure and function of camellia oleifera cake polysaccharide are not very

Method used

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  • Novel uses of camellia oleifera cake polysaccharide
  • Novel uses of camellia oleifera cake polysaccharide
  • Novel uses of camellia oleifera cake polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Preparation of Camellia oleifera cake polysaccharide:

[0028] Weigh 500g of Camellia oleifera cake and pulverize it to 80 mesh powder, add 10000mL of water, add 1750mg of cellulase (enzyme activity > 15000u / g), extract in a water bath at 50°C for 3 hours, filter with double-layer gauze, and obtain a light yellow extraction solution. The obtained extract is concentrated to 1500ml by a hollow fiber membrane separation device, so that the mass percentage of the solute in the solution is 6%. The chromatographic column of prepacked 500g polyamide is fully mixed and left to stand for adsorption for 12 hours. 4x deionized water for elution. The obtained elution solution was collected, concentrated under reduced pressure to a smaller volume, 95% ethanol five times the volume of the concentrated solution was added, fully stirred, and allowed to stand for 12 hours. Centrifuge (4500 rpm, 10 min). The precipitate was taken and vacuum freeze-dried to obtain 9.0 g of off-white...

Embodiment 2

[0034] Take 3 g of the polysaccharide obtained in Example 1, pass through a chromatographic column equipped with DEAE-52 (column height 18 cm, diameter 3 cm, column volume 85 cm), elute with 0.1 mol / L NaCl solution, collect the eluate, concentrate under reduced pressure, Water dialysis for 24 to 36 hours, freeze-dried. 129 mg of fractionated polysaccharide (named CP2) with a relative molecular weight of 8693 was obtained. Inhibition of α-glucosidase activity effect, the results are shown in Table 2.

[0035] Table 2 CP2 inhibitory effect on rat small intestinal mucosa α-glucosidase

[0036]

Embodiment 3

[0038] Get 3g of the polysaccharide obtained in Example 1, pass through a chromatographic column equipped with DEAE-52 (column height 18cm, diameter 3cm, column volume 85cm), after eluting with 0.1mol / L NaCl solution, continue to use 0.5mol / LNaCl The solution was eluted from the chromatographic column, and the eluate was collected, concentrated under reduced pressure, dialyzed in running water for 24 to 36 hours, and freeze-dried. Obtained 124mg of fractionated polysaccharide with a relative molecular weight of 12210 (named CP4). Inhibition of α-glucosidase activity effect, the results are shown in Table 3.

[0039] Table 3 CP4 inhibitory effect on rat small intestinal mucosa α-glucosidase

[0040]

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Abstract

The invention relates to novel uses of plant extract and especially relates to novel uses of camellia oleifera cake polysaccharide. The invention provides the use of camellia oleifera cake polysaccharide in inhibition of alpha-glucosidase activity and also provides the use of camellia oleifera cake polysaccharide in preparation of a drug for treating diabetes.

Description

technical field [0001] The invention relates to a new application of plant extracts, in particular to a new application of camellia oleifera cake polysaccharide. Background technique [0002] Plant polysaccharides have many biologically active functions, including immune regulation, anti-tumor, hypoglycemic, hypolipidemic, anti-radiation, antibacterial and antiviral, liver protection, etc. The extensive biological activities of plant polysaccharides have been gradually utilized by people, and their unique activities and natural sources have great potential in the application of human health protection. Therefore, how to improve the yield is the primary problem in the study of plant polysaccharides. [0003] At present, the area of ​​camellia oleifera in my country is about 45 million mu. The national annual output of camellia oleifera seeds is about 1 million tons, and 683,900 tons of camellia oleifera cake after oil extraction. [0004] The comprehensive development and ...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61P3/10
Inventor 李湘洲张胜吴志平
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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