Application of vitamin C in in-vitro expansion of number of effector memory T cells

An in vitro expansion and vitamin technology, which is applied in the application field of vitamin C to the number of effector memory T cells in vitro expansion, can solve the problems of high cost, no guarantee of clinical treatment safety, small TEM cells, etc. High security, good technical support, low cost effect

Inactive Publication Date: 2013-02-13
SUN YAT SEN UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

Since TEM cells account for a very small proportion of the total number of T cells, it is difficult to obtain them by traditional in vitro expansion methods
At present, some researchers use artificial antigens to extract cells, increase the proliferation of T cells in vitro and use them in adoptive therapy, but this technology is costly and has not yet guaranteed the safety of clinical treatment

Method used

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  • Application of vitamin C in in-vitro expansion of number of effector memory T cells
  • Application of vitamin C in in-vitro expansion of number of effector memory T cells
  • Application of vitamin C in in-vitro expansion of number of effector memory T cells

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Embodiment Construction

[0016] In order to better understand the essence of the present invention, the application of Vc on the number of effector memory T cells in vitro expansion will be described below in combination with experiments and results.

[0017] ,experiment material

[0018] Human peripheral blood was provided by Guangzhou Blood Center, and 20 blood samples from normal people were randomly selected in the experiment.

[0019] Main reagents: RPMI1640 medium was purchased from INVERTROGEN; fetal bovine serum was purchased from GIBCO; EDTA and BSA were purchased from Shanghai Sangon Biotechnology Co., Ltd.; Vc was purchased from SIGMA; lymphocyte separation medium was purchased from Tianjin Haoyang Biological Products Co., Ltd.; flow cytometry detection antibodies anti-CD4-PE, anti-CD27-FITC, anti-CCR7-PECY5, CFSE were purchased from BD Company; anti-CD45RA-ECD was purchased from BECKMAN Company; CD4 + T cell negative selection magnetic beads were purchased from BD Company.

[0020] Mai...

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Abstract

The invention discloses application of vitamin C in in-vitro expansion of number of effector memory T cells, and the optimal concentration for promoting in-vitro expansion is found. The method has the advantages of high safety, low cost and the like, and provides a good technical support for the wide development of adoptive immunotherapy.

Description

technical field [0001] The invention relates to the application of vitamin C on the expansion of effector memory T cells in vitro. Background technique [0002] Vitamin C (Vc) is a small molecule drug, which was first used clinically for the treatment of scurvy. Its antioxidant effect has been widely used. In recent years, studies have found that Vc has played a certain role in inhibiting tumor growth. , and can efficiently maintain the survival and self-renewal of stem cells. [0003] AIDS (Acquired Immune Deficiency Syndrome, AIDS) caused by HIV-1 infection is a major infectious disease threatening human health in this century. At present, highly active antiretroviral therapy (HAART) is widely used in the world to treat AIDS. HAART can control the viral load at a low level in a short period of time and improve the quality of life of AIDS patients, but it cannot completely clear the virus in the body and requires lifelong medication. HAART is expensive, has serious side e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0783
Inventor 张辉张译文罗海华刘超
Owner SUN YAT SEN UNIV
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