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ChromobacteriumpseudoviolaceumkwkjT4 biocontrol strain and application thereof

A technology of Chromobacterium violaceum and biological control strains, applied in the field of microorganisms, can solve the problems of single source of strains and weak related technologies, and achieve remarkable control effects, good development and application potential, and strong inhibitory effect

Inactive Publication Date: 2013-03-06
HUNAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the beneficial bacteria in the rhizosphere provide a new way for the biological control of rice blast, showing good application prospects, but the source of bacteria is single, and the related technology is still weak

Method used

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  • ChromobacteriumpseudoviolaceumkwkjT4 biocontrol strain and application thereof
  • ChromobacteriumpseudoviolaceumkwkjT4 biocontrol strain and application thereof
  • ChromobacteriumpseudoviolaceumkwkjT4 biocontrol strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Isolation and screening of Chromobacterium pseudoviolet kwkjT4.

[0019] The Chromobacterium pseudoviolet of the present invention, its genus name is Chromobacterium, the species name is pseudoviolaceum, and the strain name is kwkjT4. The bacterial strain is isolated and screened from the local vegetable rhizosphere soil in Xiangtan, Hunan, and specifically obtained by plate confrontation screening. The screening The method belongs to the routine experimental method in this field. The specific method and process are described as follows: Take 0.5g of vegetable rhizosphere soil (0-15cm), soak it in sterile water, shake it at 32°C for 24 hours, take the suspension, separate the bacteria by serial dilution method, select a single colony, and draw Line purification to obtain pure culture of bacteria. Rice blast fungus (06-249ZF-1, provided by Dr. Hao Zhongna, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences) was cultured ...

Embodiment 2

[0021] Example 2: In vitro bacteriostatic activity assay of Chromobacterium pseudoviolet kwkjT4 against Magnaporthe oryzae.

[0022] Cultivate the kwkjT4 strain in nutrient agar medium at 28°C for 48 hours, take 1.5mL of the bacterial solution and centrifuge at 8000r / min for 10min, get 1mL of supernatant and 30mL of PDA medium (potato sucrose medium in Example 1) Mix evenly, pour on the plate, inoculate 0.5cm rice blast fungus cake in the center of the plate after cooling, culture at 28°C, take the culture supernatant without kwkjT4 strain as the control, and measure the inhibition zone when the control grows to the edge of the culture plate size.

[0023] Relative bacteriostatic rate=(control colony expansion diameter-treatment colony expansion diameter) / control colony expansion diameter×100%. Wherein, colony extension diameter (cm)=average diameter of colony-0.5 (bacteria cake diameter).

[0024] The results showed that the inhibition rate of the kwkjT4 strain on the mycel...

Embodiment 3

[0027] Example 3: Greenhouse pot experiment of Chromobacterium pseudoviolet kwkjT4 controlling rice blast.

[0028] The rice variety is Xiushui 11, which is susceptible to Magnaporthe oryzae (06-249ZF-1). The potting test selects sterilized organic nutrient soil, 1 kg (wet weight) of soil in every pot, and sows 15 seeds to ensure 10 strong seedlings. Cultivate in a 28°C incubator for 2 days to ensure that the germination is neat, and then cultivate in a greenhouse at a temperature of 26-30°C.

[0029] Take 5×10 8 The cfu / mL kwkjT4 suspension (containing 0.1% Tween80, v / v) was sprayed on the 5-leaf-age rice seedlings, and the spray volume per pot was 2.5 mL, and the water spray was used as the control. After the treatment, it was placed in an incubator for 2 hours at a temperature of 25° C. and a humidity of 95%. Then, inoculate the Magnaporthe oryzae spore suspension (10 5 cfu / mL), cultivated in the dark for 10 hours, and cultivated in the light for 14 hours, then transfer...

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Abstract

The invention discloses a ChromobacteriumpseudoviolaceumkwkjT4 biocontrol strain capable of efficiently controlling rice blast and application thereof. The ChromobacteriumpseudoviolaceumkwkjT4 is collected at China Center for Type Culture Collection (CCTCC for short), the collection number is CCTCC M2011332, and the collection date is September 28th, 2011. The ChromobacteriumpseudoviolaceumkwkjT4 is obtained by plate confrontation; the in-vitro antibacterial activity determination and greenhouse pot experiment indicate that the ChromobacteriumpseudoviolaceumkwkjT4 has strong inhibiting action on growth of rice blast bacteria, can effectively control the rice blast, has wide antibacterial spectrum, and has antagonistic action on multiple pathogenetic fungi, such as rhizoctonia solani, wheat Pellicularia sasakii, wheat take-all bacterium and the like. The strain disclosed by the invention has the advantage of simple culture conditions, is easy to store, is environment-friendly, and has favorable development and application potential.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a biocontrol bacterial strain for preventing and treating rice blast, more specifically to a Chromobacterium pseudoviolaceum kwkjT4 for efficiently preventing and controlling rice blast and application thereof. Background technique [0002] Rice is a major food crop, and disease is an important limiting factor for its yield and quality. Rice blast caused by Magnaporthe grisea Barr. is a devastating disease of rice. The rice lost due to blast is enough to feed 60 million people every year. At present, the control of rice diseases mainly relies on chemical pesticides. Chemical pesticides have high residues, are harmful to humans and animals, pollute the environment, have environmental risks, and long-term use leads to the emergence of drug resistance. In order to improve the effectiveness of comprehensive prevention and control, it is necessary to explore new c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/01
Inventor 王海华谢宗华彭喜旭高健唐新科
Owner HUNAN UNIV OF SCI & TECH
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