Method of tissue culture and rapid propagation of viola prionantha

A tissue culture, Viola technology, applied in horticultural methods, botanical equipment and methods, cultivation and other directions, can solve the problems of few resources, early blooming Viola tissue culture has not yet been reported, etc., achieves a large reproduction coefficient, achieves sustainable The effect of using

Inactive Publication Date: 2014-03-12
SICHUAN AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] As a wild ground cover plant, Viola early blooming has not yet been developed. The source of medicine is mainly to dig wild resources, and the resources are gradually decreasing. At present, the tissue culture of Viola early blooming has not been reported, and relying solely on wild resources cannot meet the needs.

Method used

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  • Method of tissue culture and rapid propagation of viola prionantha
  • Method of tissue culture and rapid propagation of viola prionantha
  • Method of tissue culture and rapid propagation of viola prionantha

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Embodiment Construction

[0025] The present invention will be described in detail below with reference to specific embodiments.

[0026] 1.2.1 Culture medium

[0027] (1) Configuration of culture medium

[0028] Medium formulation: minimal medium (MS) supplemented with different types and concentrations of auxins and cytokinins.

[0029] The sucrose concentration is 25g / L, the agar is 6g / L, the pH value is adjusted to 6.0-6.2 with 1mol / L HCl or 1mol / L NaOH, and autoclaved at 121°C for 20min before use.

[0030] (2) Culture conditions

[0031] The culture temperature is (25±1)℃, and the light intensity is 20-30 umol / m 2 · s, Clumping shoot induction, proliferation and callus differentiation culture with a daily photoperiod of 16 h in light and 8 h in dark. For callus induction and proliferation, the cells were cultured in the dark for 24 hours every day, and then transferred to the above-mentioned photoperiod of 12 hours of daily light culture and 12 hours of dark culture after 7 days of culture. ...

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Abstract

The invention discloses a method of tissue culture and rapid propagation of viola prionantha. The method comprises: (1) a viola prionantha explant best sterilization way; (2) primary culture; (3) subculture; (4) rooting culture; and (5) hardening-seedling and transplantation. The viola prionantha cultured by the method has a great propagation coefficient, is suitable large-scale production, can solve the problem of scanty resource of the viola prionantha and realize sustainable utilization; in addition, the viola prionantha is propagated rapidly, and a large number of nursery stocks having consistent specification can be obtained in a short time, so that the method of tissue culture and rapid propagation of viola prionantha can be satisfied with the requirements of landscaping on great quantity demand of the viola prionantha, and is of great significance to gardens and medicines.

Description

technical field [0001] The invention relates to a method for plant tissue culture and rapid propagation, and belongs to the technical field of plant tissue culture and industrial rapid seedling raising. Background technique [0002] Viola prionantha Bunge (Viola prionantha Bunge) is a perennial herb of the Viola family Viola. It turns green early, grows rapidly, has strong ground covering ability, concentrated flowering period, can bloom in spring and autumn within a year, and has strong weed inhibition ability. As a wild flowering ground cover in early spring, it has good ornamental effect and garden application value. [0003] At the same time, the early opening of Viola has high medicinal value, and it is widely used in the folk for carbuncle sores, poisonous snake bites, etc. It has strong antibacterial, anti-inflammatory, heat-clearing and detoxifying effects. [0004] Early-opening Viola mainly relies on seed propagation and rhizome propagation, but the reproduction r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 张帆万雪琴刘敏钟宇刘光立张双罗兵黄金亮吴富雨
Owner SICHUAN AGRI UNIV
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