Method of tissue culture and rapid propagation of viola prionantha
A tissue culture, Viola technology, applied in horticultural methods, botanical equipment and methods, cultivation and other directions, can solve the problems of few resources, early blooming Viola tissue culture has not yet been reported, etc., achieves a large reproduction coefficient, achieves sustainable The effect of using
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[0025] The present invention will be described in detail below with reference to specific embodiments.
[0026] 1.2.1 Culture medium
[0027] (1) Configuration of culture medium
[0028] Medium formulation: minimal medium (MS) supplemented with different types and concentrations of auxins and cytokinins.
[0029] The sucrose concentration is 25g / L, the agar is 6g / L, the pH value is adjusted to 6.0-6.2 with 1mol / L HCl or 1mol / L NaOH, and autoclaved at 121°C for 20min before use.
[0030] (2) Culture conditions
[0031] The culture temperature is (25±1)℃, and the light intensity is 20-30 umol / m 2 · s, Clumping shoot induction, proliferation and callus differentiation culture with a daily photoperiod of 16 h in light and 8 h in dark. For callus induction and proliferation, the cells were cultured in the dark for 24 hours every day, and then transferred to the above-mentioned photoperiod of 12 hours of daily light culture and 12 hours of dark culture after 7 days of culture. ...
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