Preparation method and application of short-pedicel aconite root alkaloid

A technology of alkaloids and extracts, which is applied to the preparation of the alkaloid components of Artemisia chinensis, the preparation of plant extracts, and the preparation of drugs for the prevention or treatment of tumor diseases. The preparation method and anticancer activity are not disclosed, and the separation and purification are not reported, so as to achieve the effect of continuous and effective distribution and separation, avoiding solute chromatographic peak tailing, and strong antitumor activity

Inactive Publication Date: 2013-04-03
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this document only discloses a preparation scheme of the total alkaloids of Artemisia chinensis, and does not disclose its preparation method and anticancer activity
[0005] Through searching, it is found that the prior art has not reported the method of using high-speed countercurrent chromatography (HSCCC) to separate and purify the alkaloids of Artemisia chinensis, nor disclosed the anticancer activity of the alkaloids of Artemisia chinensis

Method used

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  • Preparation method and application of short-pedicel aconite root alkaloid
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  • Preparation method and application of short-pedicel aconite root alkaloid

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preparation example Construction

[0032] The preparation method of the alkaloid component of Artemisia chinensis involved in the present invention comprises the following steps,

[0033](1) Take 1 kg of Artemisia spp., crush it, soak and extract it with 1-10 times the amount (g / v) of 50%-95% ethanol for 3-6 times (12-24 h each time), and use 1-10 times The amount (g / v) of distilled water is soaked and extracted 2-4 times, the extracts are combined, recovered under reduced pressure until there is no alcohol smell, and 100-300 g of brown extract is obtained.

[0034] (2) Suspend the medicinal material extract extract with 0.5-3 times the amount of deionized water, and extract with n-hexane, chloroform, ethyl acetate, and n-butanol (1:1-1:5, v / v) respectively to obtain n-hexane alkane, chloroform, ethyl acetate, and n-butanol extracts, the solvents were recovered and dried for future use, and the weight of the obtained extract was 5-50 g.

[0035] (3) Mix n-hexane: ethyl acetate: methanol: water in a volume rati...

Embodiment 1

[0039] Example 1 Preparation of Alkaloids of Artemisia nicolae

[0040] (1) Take 1 kg of Artemisia spp., crush it, soak and extract with 1 times the amount (g / v) of 50% ethanol for 3 times (12 hours each time), and soak and extract with 1 times the amount (g / v) of distilled water for 3 times , Combine the extracts, recover under reduced pressure until there is no alcohol smell, and obtain a brown extract.

[0041] (2) Suspend the medicinal material extract extract with 0.5 times the amount of deionized water, and extract with n-hexane, chloroform, ethyl acetate, n-butanol (1:1, V / V) respectively to obtain n-hexane, chloroform, ethyl acetate Esters and n-butanol extracts were recovered and dried for later use.

[0042] (3) Mix n-hexane: ethyl acetate: methanol: water in a ratio of 5:2:3:3 by volume, let it stand overnight in a separatory funnel, and separate the upper and lower phases after the system is layered and balanced the next day, and ultrasonically Degassed for 20 ...

Embodiment 2

[0045] Example 2 Preparation of Alkaloids of Artemisia nicolae

[0046] (1) Take 1kg of Artemisia spp., crush it, soak and extract it with 10 times the amount (g / v) of 95% ethanol for 6 times (24 hours each time), and also soak and extract it with 10 times the amount (g / v) of distilled water for 3 times , Combine the extracts, recover under reduced pressure until there is no alcohol smell, and obtain a brown extract.

[0047] (2) Suspend the medicinal material extract extract with 3 times the amount of deionized water, and extract with n-hexane, chloroform, ethyl acetate, n-butanol (1:5, V / V) respectively to obtain n-hexane, chloroform, ethyl acetate Esters and n-butanol extracts were recovered and dried for later use.

[0048] (3) Mix n-hexane: ethyl acetate: methanol: water in a ratio of 9:2:7:7 by volume, let it stand overnight in a separatory funnel, and separate the upper and lower phases the next day after the system is layered and balanced, and ultrasonically Degass...

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Abstract

The invention belongs to the technical field of medicines, and relates to a method for preparing short-pedicel aconite root alkaloid and application thereof in preparation of a medicament for preventing or treating tumor diseases. Alkaloid components can be extracted from short-pedicel aconite roots in a procedure mode and in batch by adopting a system solvent method and a high-speed countercurrent chromatography (HSCCC) method; the method is simple, convenient and fast; and each alkaloid component group is identified by using thin-layer chromatography (TLC). The prepared active alkaloid ingredients are screened by adopting a methyl thiazolyl tetrazolium (MTT) method and a fluorescent staining and flow cytometry technology, have inhibiting effects on cells of liver cancer HepG2, cervical cancer Hela-60 and mice bone marrow tumor SP20, and are an excellent candidate variety of anti-tumor medicaments.

Description

technical field [0001] The present invention relates to the preparation method and application of plant extracts, in particular to the preparation method of the alkaloid component of Artemisia nigura and its use in the preparation of medicines for preventing or treating tumor diseases, and belongs to the technical field of natural medicines. Background technique [0002] Ranunculaceae Aconitum plants have been widely concerned as poisonous plants and medicinal plants. The southern part of the Hengduan Mountains in southwest my country (western Sichuan, northwestern Yunnan and eastern Tibet) is an important distribution area of ​​domestically produced Aconitum. The traditional effects of Aconitum plants in the family Ranunculaceae are dispelling wind and dampness, promoting blood circulation and removing blood stasis, warming the interior and expelling cold, etc. It is mainly used for the prevention and treatment of various plagues, such as pain relief for toothache, arthriti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/714A61P35/00
Inventor 李竣黄先菊袁琳孙东坡
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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