Spraying agent for disinfection of intensive care unit
A technology of intensive care unit and spray, which is applied in the field of clinical nursing, can solve the problems of bacterial infection and other problems, and achieve the effect of low cost, obvious disinfection effect and good safety
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Embodiment 1
[0009] Weigh 150 grams of whole-leaf green orchid, 200 grams of cassia twig, 50 grams of kansui, 80 grams of licorice, 110 grams of Fangfeng, 70 grams of small wild cocktail, 60 grams of dried ginger, 130 grams of Cordyceps 130 grams, 40 grams of Kochia chinensis, and 170 grams of cistanche. gram, 210 grams of cassia seed, 160 grams of Nongjili, 70 grams of polygala, 140 grams of aloe and 80 grams of Moutan bark, add 2000 ml of water, and place in a sealed flask. Heating in a water bath at 50° C. for 3 hours, cooling to room temperature, filtering, collecting the filtrate, concentrating to 700 ml, putting it into a nebulizer to make a spray.
experiment example 1
[0010] Experimental example 1: antibacterial test
[0011] Adopt classic filter paper method in this field to carry out bacteriostasis test, wherein:
[0012] 1. Materials
[0013] 1.1 Bacteria to be tested
[0014] Staphylococcus aureus (Staphylococcus aureus) ATCC 25923, Candida albicans (Candida albicans) ACTT 12228 were purchased from China Institute for the Control of Biological Products.
[0015] 1.2 Culture medium
[0016] Nutrient agar and nutrient broth were purchased from Beijing Zhongke Chenyu Experimental Equipment Co., Ltd.
[0017] 1.3 Test method
[0018] Inoculate Staphylococcus aureus and Candida albicans on the nutrient solution plane of the agar plate, and spread evenly and densely when inoculating. Take the aseptic circular filter paper sheet with tweezers, spray the spray prepared in Example 1 until completely wetted, and stick it on the nutrient solution plane of the agar plate inoculated with bacteria. Place the agar plate in a 37°C incubator and i...
experiment example 2
[0021] Experimental example 2: Antibacterial test in intensive care unit
[0022] Indoor area is equal to 30m 2 In the intensive care unit, 100 milliliters of sprays prepared in Example 1 were evenly sprayed. 12 hours, 24 hours, and 48 hours after spraying, use the plate sedimentation method to sample, set 3 sampling points, and take 3 points on a diagonal line, that is, 1 point in the middle and 1 point at each end 1m away from the wall , and repeat 10 times. After sampling, culture the culture medium in a 35°C incubator for 48 hours, observe the number of bacterial colonies on the culture medium in a bright place, and calculate the number of bacteria according to the following formula. The amount of bacteria in the air (cfu / m 3 ) = average number of colonies per dish × 50000 / plate exposure time × plate area. All bacteria were identified in accordance with the "National Clinical Testing Procedures".
[0023] 12 hours, 24 hours, 48 hours after spraying, the number of bac...
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