Marine functional food capable of inhibiting prostatic cancer and preparation method of marine functional food

Abstract

The invention belongs to marine functional food and particularly relates to marine functional food capable of inhibiting prostatic cancer. The marine functional food belongs to a squid ink polypeptide active matter prepared through extracting squid ink by an acid method. A specific method comprises the steps that hydrochloric acid is added into the preprocessed squid ink, the leaching is carried out under the conditions that the hydrochloric acid concentration is 0.02 to 0.03 mol / L, the acid lifting material liquid ratio (the volume ratio of double distilled water to raw material squid ink) is 1:(1-2), the acidolysis time is 2 to 3 hours, the acidolysis temperature is 35 DEG C to 40 DEG C, finally, the leaching liquid is centrifuged to obtain clear liquid, and the obtained clear liquid is the coarse squid ink polypeptide active matter. Rich marine biological squid ink is adopted, the squid ink polypeptide is purified by methods of acid method extraction, concentration and the like, in addition, the optimal acidolysis process is obtained through an orthogonal test, and the most squid ink polypeptide active matters are obtained. CCK-8 method detection shows that the squid ink polypeptide has the obvious proliferation inhibition effect on DU-145 and PC-3 cells of the prostatic cancer, can inhibit the DU-145 and PC-3 cell proliferation and can induce the apoptosis of the cells, so the prostatic cancer is inhibited.

Description

technical field [0001] The invention belongs to marine functional foods, in particular to a marine functional food capable of inhibiting prostate cancer and a preparation method thereof. Background technique [0002] In European and American countries, the incidence of prostate cancer (PCa) is the first among male malignant tumors, and the mortality rate is second only to lung cancer. In recent years, with the aging of the population in my country, the incidence of PCa has also increased significantly, and it has become a major disease affecting the health of elderly men. Although the incidence of PCa is lower than that of European and American countries, the mortality rate is much higher than that of European and American countries. . For androgen-independent prostate cancer, there is still no effective treatment, and it is easy to relapse, and anti-androgen therapy is ineffective. Therefore, the lack of effective treatment for androgen-independent prostate cancer is the b...

AI Technical Summary

Problems solved by technology

For androgen-independent prostate cancer, there is still no effective treatment, and it is easy to relapse, and anti-androgen therapy is ineffective

Therefore, the lack of effective treatment for androgen-independent prostate cancer is the biggest problem in the clinical treatment of prostate cancer. Researchers have been looking for active substances that can effectively treat androgen-independent prostate cancer.