Breeding method of insect-resistant big-stigma three-line sterile line

A three-line sterile line, stigma technology, applied in the directions of botanical equipment and methods, applications, plant genetic improvement, etc.

Active Publication Date: 2013-06-05
福建亚丰种业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there are few studies on the large stigma male sterile line of rice, especially the insect resistant large stigma male sterile line, which has not been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1) IR40 was used as the parent of resistance to Chilo borer (P 1 ), with IR26 as the rice planthopper-resistant parent (P 2 ), with GST-1 as the large stigma parent (P 3 ), for hybridization P 1 / P 3 and P 2 / P 3 , respectively planting P 1 / P 3 Hybrid F 1 (F 1 -1) and P 2 / P 3 Hybrid F 1 (F 1 -2);

[0021] 2) In Hybrid F 1 F 1 -1 / F 1 -2, mix and harvest after maturity and plant hybrid combination F 1 -1 / F 1 Hybrid F of -2 1 Generation, mixed harvest F at maturity 1 Seeds from generation plants (F 2 generation);

[0022] 3) Planting 2) the resulting hybrid F 2 Generation seeds, with a population of more than 2,000 plants, identify the resistance of rice planthoppers at the seedling stage, select rice seedlings with high resistance to rice planthoppers for transplanting, and identify the resistance of rice stem borer at the tillering stage, and select rice plants without pests. During the flowering stage, the size of the stigma of the spikelets was...

Embodiment 2

[0030] 1) IR36 was used as the parent of resistance to Chilo borer (P 1 ), with IR46 as the rice planthopper-resistant parent (P 2 ), with GST-1 as the large stigma parent (P 3 ), for hybridization P 1 / P 3 and P 2 / P 3 , respectively planting P 1 / P 3 Hybrid F 1 (F 1 -1) and P 2 / P 3 Hybrid F 1 (F 1 -2);

[0031] 2) In Hybrid F 1 F 1 -1 / F 1 -2, mix and harvest after maturity and plant hybrid combination F 1 -1 / F 1 Hybrid F of -2 1 Generation, mixed harvest F at maturity 1 Seeds from generation plants (F 2 generation);

[0032] 3) Planting 2) the resulting hybrid F 2 Generation seeds, with a population of more than 2,000 plants, identify the resistance of rice planthoppers at the seedling stage, select rice seedlings with high resistance to rice planthoppers for transplanting, and identify the resistance of rice stem borer at the tillering stage, and select rice plants without pests. During the flowering stage, the size of the stigma of the spikelets was...

Embodiment 3

[0040] 1) IR40 was used as the parent of resistance to Chilo borer (P 1 ), with IR46 as the rice planthopper-resistant parent (P 2 ), with GST-1 as the large stigma parent (P 3 ), for hybridization P 1 / P 3 and P 2 / P 3 , respectively planting P 1 / P 3 Hybrid F 1 (F 1 -1) and P 2 / P 3 Hybrid F 1 (F 1 -2);

[0041] 2) In Hybrid F 1 F 1 -1 / F 1 -2, mix and harvest after maturity and plant hybrid combination F 1 -1 / F 1 Hybrid F of -2 1 Generation, mixed harvest F at maturity 1 Seeds from generation plants (F 2 generation);

[0042] 3) Planting 2) the resulting hybrid F 2 Generation seeds, with a population of more than 2,000 plants, identify the resistance of rice planthoppers at the seedling stage, select rice seedlings with high resistance to rice planthoppers for transplanting, and identify the resistance of rice stem borer at the tillering stage, and select rice plants without pests. During the flowering stage, the size of the stigma of the spikelets was...

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PUM

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Abstract

The invention discloses a breeding method of an insect-resistant big-stigma three-line sterile line, belonging to the technical field of rice insect-resistant breeding. The breeding method is characterized in that a three-line maintainer line B is taken as a recurrent parent, a chilo-suppressalis-resisting parent, a rice-planthopper-resisting parent and a big-stigma parent are subjected to hybridization, backcross and insect-resistant screening to polymerize big stigma, chilo-suppressalis-resisting and rice-planthopper-resisting genes in the three-line maintainer line B, and then the highly chilo-suppressalis-resisting and planthopper-resisting big-stigma three-line sterile line is formed through hybridization and backcross of the line B and a corresponding line A. The sterile line is highly resistant to rice planthopper and chilo suppressalis, is easy to expose because of large stigma of glumous flowers, and is high in exposure rate of the stigma, outcrossing maturing rate and seed production yield; and the seed production yield and the insect-resistant level of hybrid rice can be improved when the insect-resistant big-stigma three-line sterile line is used for preparing the hybrid rice.

Description

technical field [0001] The invention specifically relates to a method for selecting an insect-resistant large stigma three-line sterile line, and belongs to the technical field of rice insect-resistant breeding. Background technique [0002] Rice is China's largest food crop. The total output of rice in China accounts for more than 40% of the total output of food crops. A bumper rice harvest is crucial to ensuring my country's food security. In recent years, my country's rice pests and diseases outbreaks are frequent, the damage is serious, and the resistance develops rapidly, and the yield reduction phenomenon caused by pests and diseases is serious. According to the analysis and prediction of the National Agricultural Technology Extension Service Center, the area of ​​rice diseases and insect pests is expected to occur 1.5 billion mu in 2012. Among them, the insect pests are mainly rice planthoppers, rice stem borers and rice leaf rollers. 100 million mu times, and the ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 章清杞郑植陈志伟
Owner 福建亚丰种业有限公司
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